Sema YILDIZ, Hasan ÇEÇE, Zeynep AKTI, İhsan KAYA, Hakim ÇELİK, Hale ÇAKIR, Abdullah TAŞKIN, Nurten AKSOY

Bilgisayarlı Tomografinin oksidatif stres parametreleri üzerine etkisi

Amaç: Bu çalışmanın amacı, kontrastsız bilgisayarlı tomografi BT sonrası erken dönem serum oksidatif stres belirteçlerinden olan seruloplazmin, lipit hidroperoksit LOOH ve total serbest sülfidril gruplarındaki -SH değişikliklerin saptanmasıdır.Materyal ve metod: Çalışmaya spiral BT cihazı Toshiba X-vision ,Tokyo, Japonya ile kontrastsız kraniyal tomografi çekilen 28 hasta dahil edildi. Venöz kan örneklemleri tetkik öncesi grup I ve kontrastsız BT tetkikinden hemen sonra grup II elde edildi. Serum seruloplazmin seviyeleri otomatik kolorimetrik metotla, serum LOOH seviyeleri ferröz iyon oksidasyonu 'xylenol orange' FOX-2 yöntemiyle çalışıldı. Sülfidril gruplarının konsantrasyonu, serbest sülfidril grubu olarak redükte glutatyonun spektrofotometrik olarak hesaplanmasıyla elde edildi. Her olguya ait iki grubu içeren serum oksidatif stress parametrelerinin kıyaslanmasında bağımlı örneklem t- testi kullanıldı.Bulgular: Serum LOOH 4,71±1,43'e karşı 5,13±1,53 μmol tBLOOH/L, p=0,179 ve –SH seviyeleri 0,311±,029'e karşı 0,305±0,025 mmol/L, p=0,084 yönünden grup I ve grup II arasında anlamlı fark saptanmadı. Grup II'nin serum seruloplazmin seviyesi grup I'e göre anlamlı olarak düşük saptanmıştır. 741,7±70,7'e karşı 757,4±63,6 U/L, p=0,015 . Sonuç: BT'nin bilinen diğer etkilerinin yanında, bu çalışma ile kontrastsız BT sonrası erken dönemde serum LOOH ve –SH düzeyleri değişmese de serum seruloplazmin düzeylerinde belirgin azalma saptanmıştır ve bu konuda daha uzun takip sürelerini içeren ileri çalışmalara ihtiyaç vardır

Anahtar Kelimeler:

Bilgisayarlı tomografi, oksidatif stres, lipit hidroperoksit, seruloplazmin, total serbest sülfidril grupları

Impact of Computed Tomography on oxidative stress

Background: We aimed to assess the impact of computed tomography CT on serum oxidative stress markers of ceruloplasmin, lipid hydroperoxide LOOH and total free sulfhydryl groups –SH in patients undergoing non contrastenhanced CT.Methods: The study included 28 patients undergoing non contrast-enhanced cranial CT with spiral CT Toshiba Xvision , Tokyo, Japan . Venous blood samples were withdrawn at baseline Group I , and after non contrast-enhanced CT Group II . Serum ceruloplasmin levels were determined with an automatic colorimetric method, whereas serum LOOH levels were determined by the ferrous ion oxidation-xylenol orange FOX-2 method based on a known principle of the oxidation of Fe II to Fe III by lipid hydroperoxides, under acidic conditions. The concentration of sulfhydryl groups was calculated spectrophotometrically using reduced glutathione as the free sulfhydryl group. Comparison of serum oxidative stress markers among two groups of samples from each case were performed with paired samples t-test.Results: Serum LOOH 4.71±1.43 vs. 5.13±1.53 μmol tBLOOH/L, respectively, p=0.179 and –SH levels .311±.029 and .305±.025 mmol/L respectively, p=0.084 were not significantly different between Groups I and II. Serum ceruloplasmin was significantly decreased in Group II compared to Group I 741.7±70.7 vs.757.4±63.6 U/L respectively, p=0.015 . Conclusions: Besides the previous studies revealing deleterious effects of CT, the present study has shown decreased serum ceruloplasmin levels [besides unchanged serum LOOH and –SH levels] after performing non contrast-enhanced CT compared to baseline that might reveal a novel untoward effect of CT which should be reinforced with further researches

Keywords:

Ceruloplasmin, computed tomography, lipid hydroperoxide, oxidative stress, total free sulfhydryl groups,

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