Granülosit-makrofaj koloni-stimülan factor uygulanması bozulmuş flep iyileşmesinde doku CD4/CD8 oranını arttırıyormu?

Amaç: Yara iyileşmesinde T hücre bağımlı immün yanıtın sağlıklı olması esansiyel öneme sahiptir. Glukokortikoidler T hücre proliferasyonunu inhibe ederek hücresel yanıtı bozmalarına karşın bir proinflamatuar sitokin olan granülositmakrofaj koloni stimülan faktör GM-CSF dolaşımdaki CD4/CD8 oranını arttırarak immün stimülasyon gerçekleşir. Bu çalışmada bozulmuş yara iyileşmesi modelinde lenfositlerin rolünün immunhistokimyasal olarak ortaya konulması amaçlanmıştır. Gereç - Yöntem: 72 adet Wistar albino rat 200 – 240 g arası üç gruba ayrılmıştır: I Grup Serum fizyolojik + flep kaldırma ; II Grup Sistemik metilprednizolon uygulaması + serum fizyolojik + flep kaldırma ; III Grup Sistemik metilprednizolon uygulaması + GM-CSF + serum fizyolojik + flep kaldırma . Doku örenkleri % 10’ luk formalinde fikse fiksasyonun ardından parafine gömüldükten sonra kullanılmıştır. Örneklerden alınan kesitler CD4 ve CD8 monoklonal antikorları ile boyandıktan sonra immünohistokimyasal olarak incelenmiştir. Bulgular: I Gruptaki kesitlerden daha kötü olmasına karşılık CD4 ve CD8 ile boyanma III Gruba ait histolojik kesitlerde II Gruba ait olanlardan daha iyi boyanmıştır P

Anahtar Kelimeler:

Flep iyileşmesi, immünohistokimya, GM-CSF, CD4, CD8

Does priming granulocyte-macrophage colony-stimulating factor increase tissue CD4/CD8 ratioin impaired flap healing?

ABSTRACT Background: The presence of an intact T-cell immune system is essential for a normal wound healing. Glucocorticoids impair cellular immune response by inhibiting T-cell proliferation, but granulocytemacrophage colony-stimulating factor GM-CSF which is a proinflammatory cytokine leads to immune stimulation by increasing the levels of circulating CD4 and CD8 cells. The purpose of this study was to elucidate the role of lymphocytes in impaired wound healing model by using immunohistochemistry. Methods: 72 Wistar albino rats 200 to 240 g were divided into three groups: Group I given saline + flap elevation ; Group II given systemic methylprednisolone + saline + flap elevation ; Group III given systemic methylprednisolone + GM-CSF + flap elevation . Samples fixed in 10 % formaline and embedded in parafin were used. The sample sections were evaluated immunohistochemically using monoclonal antibodies for CD4 and CD8. Results: CD4 and CD8 staining of histologic sections in GM-CSF-treated group III were better than those of group II, although they all remained worst than those of group I p> 0.05 . Conclusions: The results obtained in this study, suggest the possiblity to abrogate the immunesuppressive effects of methylprednisolone by GM-CSF injection. To restore lymphocyte function using proinflammatory cytokines is important for wound healing and prevention of immunosuppression

Keywords:

Flap healing, immunohistochemistry, GM-CSF, CD4, CD8,

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