Deneysel Diyabette Deri Hasarına Karşı Propolisin İyileştirici Etkilerinin Araştırılması

Özet Amaç: Bu çalışmada deneysel Diabetes Mellitus’un dermal doku üzerinde oluşturduğu hasara karşı propolisin muhtemel olumlu etkilerini histopatolojik parametreler ile araştırmaktır. Materyal ve Metod: Çalışmamızda 24 adet 10-12 haftalık Wistar Albino türü erkek sıçanlar Kontrol, Propolis 100mg, DM ve DM+Propolis (100mg) olarak rastgele 4 gruba ayrıldı. 0.1 Molar (Ph: 4,5) sitrat tamponunda çözülen tek doz 60 mg/kg Streptozotosin (inroperitoneal) ile deneysel diyabet oluşturuldu. Deney süresinin bitiminde (28. Gün) tüm gruplar sakrifiye edildi ve elde edilen dermal dokularda Hematoksilen&Eozin ve Masson Trikrom boyamaları ile histopatolojik değerlendirme yapıldı. Bulgular: Yapılan histopatolojik değerlendirmede; kontrol ve Propolis 100mg gruplarına ait deri örneklerinin normal morfolojik yapıda olduğu izlendi. DM grubunda çok tabakalı epitelde incelme, dejenerasyon, dermis tabakasında yer alan bağ dokusunda ayrılma ve azalma gözlenirken DM+Propolis 100 mg grubunda ise bu bulgularda belirgin şekilde bir iyileşme izlendi. Sonuç: Bu çalışma, Propolis 100mg dozunun biyolojik özelliklerin diyabetin dermal dokuda meydana getirdiği cilt hasarını morfolojik düzeyde önleyebileceğini göstermiştir. Anahtar kelimeler: Deri, Diabetes Mellitus, Propolis, Histopatoloji, Rat

Anahtar Kelimeler:

Deri, Diabetes Mellitus, Propolis, Histopatoloji, Rat

Investigation of The Ameliorative Effects of Propolis Against Skin Damage in Experimental Diabetes.

Background: The purpose of this study was to investigate the possible positive effects of propolis against the damage caused by experimental Diabetes Mellitus on the dermal tissue with histopathological parameters. Methods: Twenty-four male Wistar Albino rats aged 10-12 weeks were randomly assigned into four groups as; Control, Propolis 100mg, DM and DM+Propolis (100mg). Experimental diabetes was induced with a single dose of 60 mg/kg streptozotocin (intraperitoneally) dissolved in 0.1 Molar (Ph: 4.5) citrate buffer. At the end of the experiment period (Day 28), all groups were sacrificed and histopathological evaluation was performed on the dermal tissues by Hematoxylin&Eosin and Masson Trichrome staining. Results: In histopathological evaluation; normal morphological structure were observed in the skin samples of the control and Propolis 100mg groups. Thinning of the multilayered epithelium, degeneration, separation and decrease in the connective tissue in the dermis layer was observed in DM group, but these findings were significantly improved in the DM+Propolis 100 mg group. Conclusions: The present study showed that the biological properties of Propolis 100mg dose may prevent skin damage caused by diabetes at the morphological level in dermal tissue. Key words: Skin, Diabetes Mellitus, Propolis, Histopathology, Rat

Keywords:

Skin, Diabetes Mellitus, Propolis, Histopathology, Rat,

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