Boya infüzyon tekniği ile bazı Prunus sürgünlerinin ksilem su akış hızının belirlenmesi

Ksilem demetindeki su hareketi hidrolik sistemi temsil etmektedir. Hidrolik sistem, çevresel streslerin neden olduğu emboli (kavitasyon) ile karşılaşabilmektedir. İzleyici boyalar ksilem içinde dağılabilmekte ve boya infüzyon adı verilen teknik ile dokuyu bölümlere ayırarak izlenebilmektedir. Mevcut çalışma, kiraz ve şeftali ağaçlarının sürgünlerinde su iletim yollarını izlemek için asit fuksin boya infüzyon tekniği için en uygun koşulları belirlemiştir. Gisela 5 üzerine aşılı 18 yaşındaki 0900 Ziraat kiraz çeşidi ve Garnem ve GF 677 üzerine aşılı 2 yaşındaki Rich May şeftali çeşidinin sürgünleri çalışmada kullanılmıştır. Ksilem işlevi ve ksilem su akış hızı (VXW) boya infüzyon tekniği ile değerlendirilmiştir. Sürgünlerde asit fuksin boyamasının yoğunluğu, boya konsantrasyonu ile artmıştır. Kiraz ağaçlarında, VXW zamanla azalmış ve 120 dakika en düşük VXW değere olmuştur. Şeftalide, GF 677'nin VXW'si Garnem'e kıyasla daha fazla bulunmuştur. Çalışma, boya infüzyon tekniğinin su iletim yollarının ve ksilem işlevinin görselleştirilmesi için güvenilir olduğunu göstermiştir. Ayrıca bu teknik, ağaçların tomurcuk dormansilerinin kırılmalarında ve stres çalışmalarında kullanılabilir.

Anahtar Kelimeler:

Asit fuksin, şeftali, kiraz, su akışı, ksilem

Determination of xylem water flow velocity of some Prunus shoots by dye infusion technique

Water movement in xylem vessel presents hydraulic system. The hydraulic system may encounter embolism (cavitation) caused by environmental stresses. Tracer dyes are distributed within the xylem and can be monitored by sectioning the tissue called dye infusion technique. The current experiment determined the optimal conditions for acid fuchsin dye infusion technique to monitor the water-conducting pathways in shoots of sweet cherry and peach trees. Shoots from 18-year-old sweet cherry cultivar 0900 Ziraat grafted onto Gisela 5 and 2-year-old peach cultivar Rich May grafted onto Garnem and GF 677 were used in the experiment. Xylem functionality and xylem water flow velocity (VXW) were evaluated by dye infusion technique. The intensity of acid fuchsin staining in the shoots increased with dye concentration. In sweet cherry trees, VXW decreased with the time course and 120 mn had the lowest VXW value. In peach, VXW of GF 677 was found further compared to Garnem. The experiment showed that the dye infusion technique is reliable for the visualization of water-conduction pathways and xylem functionality. Furthermore, the technique can be used in the studies of bud dormancy break of trees and stresses.

Keywords:

Acid fuchsin, Peach, sweet cherry, water flow, xylem,

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