Investigation on Some Biological Activities of Different Parts of Vincetoxicum hirundinaria Medik.

In this study, it was aimed to determine the antimicrobial, antibiofilm activities and synergistic effects of ethanol extracts obtained from above-ground, root, seed and seed coat of Vincetoxicum hirundinaria Medik. Antimicrobial activity was performed against 7 bacterial and 3 fungal reference strains by microdilution method and minimum inhibition concentrations (MIC) were determined. The crystal violet method was applied to determine the prevention of biofilm formation and inhibition of preformed biofilm activities on Pseudomonas aeruginosa biofilm. In addition, the synergistic effects of aerial part and seed extracts against both Escherichia coli and Enterococcus faecalis were examined by the microdilution checkerboard method. According to the antimicrobial results extracts had moderate to low efficacy against bacterial and yeast strains. The synergy test showed that the aerial and seed extracts had additive effect against both E.coli and E.faecalis. The extracts also showed the potential to inhibit biofilm formation and preformed biofilms. Especially root and seed pod extracts showed strong antibiofilm activity. In conclusion, the literature search indicated that the antimicrobial and antibiofilm activities of V. hirundinaria was evaluated for the first time in the current study, therefore; our findings provide important preliminary data to the literature in terms of antibiofilm activity of V. hirundinaria.

Anahtar Kelimeler:

Antimicrobial, Antibiofilm, Synergy Test, Vincetoxicum hirundinaria

Investigation on Some Biological Activities of Different Parts of Vincetoxicum hirundinaria Medik.

Keywords:

Antimicrobial, Antibiofilm, Synergy Test, V. hirundinaria,

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Bjarnsholt T. The role of bacterial biofilms in chronic infections. APMIS Suppl. 2013;136:1-51. doi: 10.1111/apm.12099
Berlanga M and Guerrero R. Living together in biofilms: the microbial cell factory and its biotechnological implications. Microb Cell Fact. 2016;15(1):165. doi: 10.1186/s12934-016-0569-5.
Whitchurch CB, Tolker-Nielsen T, Ragas PC, and Mattick JS. Extracellular DNA required for bacterial biofilm formation, Science. 2002;295(5559):1487. doi: 10.1126/science.295.5559.1487.
Melandera RJ, Basaka AK, Melandera C. Natural Products as Inspiration for the Development of Bacterial Antibiofilm Agents. Nat Prod Rep. 2020;37(11):1454–1477. doi:10.1039/d0np00022a.
Macia MD, Rojo-Molinero E, Oliver A. Antimicrobial susceptibility testing in biofilm-growing bacteria. Clin Microbiol Infect. 2014;20(10):981-90. doi: 10.1111/1469-0691.12651.
Wolcott RD, Rhoads DD, Bennett ME, Wolcott BM, Gogokhia L, Costerton JW, Dowd SE. Chronic wounds and the medical biofilm paradigm. J Wound Care. 2010;19(2):45-6. doi: 10.12968/jowc.2010.19.2.46966.
Rabin N, Zheng Y, Opoku-Temeng C, Du Y, Bonsu E, Sintim HO. Biofilm formation mechanisms and targets for developing antibiofilm agents. Future Med Chem. 2015;7(4):493-512. doi: 10.4155/fmc.15.6.
Del Pozo JL. Biofilm-related disease. Expert Rev Anti Infect Ther. 2018;16(1):51-65. doi: 10.1080/14787210.2018.1417036.
Guzzo F, Scognamiglio M, Fiorentino A, Buommino E, D'Abrosca B. Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms. Molecules. 2020;25(21):5024. doi: 10.3390/molecules25215024.
Lagha R, Abdallah FB, Al-Sarhan BO, Al-Sodany Y. Antibacterial and Biofilm Inhibitory Activity of Medicinal Plant Essential Oils Against Escherichia coli Isolated from UTI Patients. Molecules. 2019;24(6):1161. doi: 10.3390/molecules24061161.
DiTommaso A, Lawlor FM, Darbyshire SJ. The biology of invasive alien plants in Canada 2. Cynanchum rossicum (Kleopow) Borhidi (=Vincetoxicum rossicum (Kleopow) Barbar.) and Cynanchum louseae (L.) Kartesz&Gandhi (= Vincetoxicum nigrum (L.) Moench). Can J Plant Sci. 2004;85(1): 243-263.
Güven S, Makbul S, Coskuncelebi K, Pınar NM. Pollinarium morphology of Vincetoxicum (Apocynaceae: Asclepiadoideae) in Turkey. Phytotaxa. 2015;230(1): 22-38
Sliumpaite I, Murkovic M, Zeb A, Venskutonisa PR. Antioxidant properties and phenolic composition of swallowwort (Vincetoxicum lutea L.) leaves. Ind Crops Prod. 2013;45:74-82.
Mansoor A, Ibrahim MA, Zaidi MA, Ahmed M. Antiprotozoal activities of Vincetoxicum stocksii and Carum copticum. Bangladesh J Pharmacol. 2011;6(1): 51-54.
Browicz K. Vincetoxicum N.M. Wolf. In: Davis PH, eds. Flora of Turkey and the East Aegean Islands. Edinburgh, Scotland; 1978. p. 163-173.
Slapšytė G, Dedonytė V, Adomėnienė A, Lazutka JR, Kazlauskaitė J, Ragažinskienė O, Venskutonis PR. Genotoxic properties of Betonica officinalis, Gratiola officinalis, Vincetoxicum luteum and Vincetoxicum hirundinaria extracts. Food and Chemical Toxicology. 2019;134:110815. doi: 10.1016/j.fct.2019.110815. Epub 2019 Sep 11.
Lavault M, Richomme P, Bruneton J. Acetophenones and new pregnane glycosides from the roots of Vincetoxicum hirundinaria. Fitoterapia. 1999;70(2): 216-220.
Pavela R. Antifeedant activity of plant extracts on Leptinotarsa decemlineata Say. and Spodoptera littoralis Bois. larvae. Ind Crops Prod. 2010;32(3): 213-219.
Lavault M, Richomme P, Bruneton J. New phenanthroindolizidine N-oxides alkaloids isolated from Vincetoxicum hirundinaria Medik. Pharm Acta Helv. 1994;68(4): 225-227.
Coassini-Lokar L, Poldini L. Herbal remedies in the traditional medicine of the Venezia Giulia Region (North East Italy). J Ethnopharmacol. 1988;22(3): 231-279.
Baytop T. Türkiye'de Bitkiler ile Tedavi Geçmişte ve Bugün. İlaveli 2. Baskı; İstanbul Üniversitesi Eczacılık Fakültesi, Nobel Tıp Kitap Evleri, İstanbul, Türkiye 1984.
Güzel S, Ülger M, Kahraman A. Chemical composition and some biological activities of Salvia longipedicellata Hedge mericarps. Chemistry of Natural Compounds. 2020;56(5): 788-792.
Woods GL, Washington JA. Antibacterial susceptibility tests: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of clinical microbiology. 6th ed, Washington, DC: American Society for Microbiology; 1995. p. 1327–41.
Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing: general principles and contemporary practice. Clin Infect Dis. 1998;26:973–80.
O’Toole GA. Microtiter dish biofilm formation assay. J Vis Exp 30;(47):2437. doi: 10.3791/2437.
H. Zhong, Z. Xie, H. Wei, S. Zhang, Y. Song, M. Wang, and Y. Zhang. Antibacterial and Antibiofilm Activity of Temporin-GHc and Temporin-GHd Against Cariogenic Bacteria, Streptococcus mutans. Front Microbiol. 2019;10:2854. doi: 10.3389/fmicb.2019.02854.
Moody J. Synergism testing: broth microdilution checkerboard and broth microdilution methods. In: Garcia LS, eds. Clinical Microbiology Procedures Handbook. Washington, DC: ASM Press; 2010. P. 5.12.11-5.12.23.
Chin NX, Weitzman I & Della-Latta P. In vitro activityof fluvastatin, a cholesterol-lowering agent, and synergy with fluconazole and itraconazole against Candida species and Cryptococcus neoformans. Antimicrobial Agents and Chemotherapy. 1997;41(4), 850-852.
Güzel S, Ülger M, Aslan G, Kökdil G. Evaluation of antimicrobial activity of five Vincetoxicum taxa growing in Turkey. Marmara Pharmaceutical Journal 2018;22(3), 365-373.
Güzel S, Ülger M, Özay Y, Yumrutaş Ö, Bozgeyik I, Sarıkaya Ö. Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata tohumlarının antimikrobiyal ve antiproliferatif aktiviteleri. Lokman Hekim Dergisi 2019;9: 367-375.
Güzel S, Pavela R, Kökdil G. Evaluation of antifungal effect of different polarity extracts from five Vincetoxicum taxa against Aspergillus fumigatus. International Anatolian Academic Online Journal/Health Science. 2015;3(2): 1-9.
Güzel S, Pavela R, Kökdil G. Phytochemistry and antifeedant activity of root extracts from some Vincetoxicum taxa against Leptinotarsa decemlineata and Spodoptera littoralis. JBiopest. 2015;8(2):128-140.
Güzel S, Pavela R, İlçim A, Kökdil G. Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata. Marmara Pharm J. 2017;21(4):872-880.