Farmasötik Formülasyonlarda Allopurinol’ün Sıfırıncı Dereceden ve Birinci Dereceden Türev Spektrofotometrisi Yöntemleriyle Kantitatif Analizi
Bu çalışmada tabletlerde Allopurinol’ün kantitatif analizi için doğrudan absorbans ölçümü ve birinci türev spektrofotometrisi yöntemlerini kapsayan iki farklı spektrofotometrik yöntem geliştirildi. Allopurinol’ün analizi için 245.0 nm dalga boyunda doğrudan absorbans ölçümleri yapılarak kalibrasyon eğrisi elde edildi. Birinci dereceden türev spektrofotometrisi yönteminde 257.6 nm de dalga boyunda dA/dλ değerleri kullanılarak kalibrasyon denklemi hesaplandı. Allopurinol’ün miktar tayini için her iki yöntemin uygulanmasında doğrusal çalışma aralığı olarak 4.0-36.0 μg/mL bulunmuştur. Hazırlanan test numunelerinin analizi ve standart ekleme tekniği kullanılarak önerilen analitik yöntemler valide edildi. Analizler, ilgili ilacın miktar tayini için kullanılan yöntemlerin kesin, doğru ve güvenilir olduğunu gösterdi.
Anahtar Kelimeler:
Spektrofotometri, Kantitatif Analiz, Allopurinol, Tablet
Zero-Order Spectrophotometry and First- Order Derivative Spectrophotometry for the Quantitative Estimation of Allopurinol in a Pharmaceutical Formulation
In this study, two different spectrophotometric methods, direct absorbance measurement method and first derivative spectrophotometry were proposed for the quantitative analysis of allopurinol in tablets. In the application of direct absorbance measurement to the analysis of allopurinol, calibration curve was obtained by measuring the absorbance values at 245.0 nm in the zero-order spectra. In first derivative method, calibration equation was computed by using the dA/dλ values at 257.6 nm. Linearity range for both methods were found between 4.0-36.0 μg/mL for the analysis of allopurinol. The proposed methods were checked by analyzing external test samples containing allopurinol and using standard addition samples. Analysis results showed that the applied methods were precise, accurate and reliable for the quantitation of the related drug.
Keywords:
Spectrophotometry, Quantitative analysis, Allopurinol, Tablets,
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- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 1981
- Yayıncı: Hacettepe Üniversitesi Eczacılık Fakültesi Dekanlığı
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