Yüzeyde Gösterilen Enzimler İçin Bakteriyel Bir Mekanizma

Biyomateryal temelli protein taşıma sistemleri birçok biyotıp uygulamasında kullanılmak üzere iyileştirildi. Büyük başarılarına rağmen yenilikçi ve karar verme mekanizmasına sahip sistemlere protein taşınmasında hala ihtiyaç duyulmaktadır. Bu çalışmada protein salınımı ve taşınması için hücresel bir sistem tasarlanmıştır. Bu sistemler sadece biyotıp uygulamalarında değil, aynı zamanda diğer biyokimyasal sistemler için de uyarlanabilir. Bu bağlamda bir Escherichia coli oto-taşıyıcı protein, Ag43 hücre zarında gösterilmek üzere tasarlanmıştır. Alkali fosfataz proteinli bu sistem kullanılarak Ag43 ve özellikli TEV proteaz kesme bölgesi taşıyan ALP’nin birleşmesi gösterilmiştir. Enzimin aktif formunun hücre yüzeyinden salgılanan TEV proteaz ile iletişimi sonucunda salındığı açıklanmıştır ve bu makalede kontrollü enzim taşıma sistemini sağlayan bir hücresel mekanizma önerilmiştir.

A Bacterial Machinery for Surface Displayed Enzymes

Biomaterial based protein delivery systems have been utilized for many applications in biomedicine. Despite their great success, there is a need to develop innovative living, decision making systems for protein delivery. In this context, here, a cellular system is proposed for protein release and delivery. Such systems can be used not for biomedical purposes but also for other biochemical applications. In this regard a Escherichia coli autotransporter protein, Ag43 was engineered to display on its cell membrane. Using this system alkaline phosphatase protein is displayed on the cell surface as a fusion of Ag43-ALP which is also carrying a specific TEV protease excision site. It was shown that the active from of the enzyme was released upon its interaction with TEV protease from the cell surface. In this study a cellular machinery is proposed to be used as a controlled enzyme delivery system.

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