The effect of the neurosecretory material in the median neurosecretory cells on Egg development of the endoparasitic Pimpla turionellae L. (Hymenoptera: ichneumonidae)

The purpose of this study is to examine the effects of the neurosecretory material (NSM) in median neurosecretory cells (MNC) on reproduction of endoparasitic Pimpla turionellae L.. For this purpose, the egg maturation and amount of the NSM in MNC of the insects were examined by the serial cross sections of the brain. Egg maturation was determined by measuring the terminal oocyte length in the ovariole. The sections of insect brains were stained by paraldehyde fuchsin to determine the amount of the NSM in the neurosecretory granules of the MNC. The amount of the NSM in MNC was at a certain level in the first day of egg development. While terminal oocyte was reaching the maximum length, amount of NSM in MNC decreased the minumum level. During the egg laying phase, the amount of the NSM in MNC reached the maximum level. These observations give us the idea that the neurosecretory material in median neurosecretory cell of this insect may be related to the egg development.

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1. Lea, A.O., Thomsen, E., Size idependent secretion by the corpus ailatum of Calliphora erythrocephala. J. Insect Physiol., 15, 477, 1969.
2. Meola, R., Lea, A.O., Independence of paraldehyde-fuchsin staining of the corpus cardiacci and the presence of the neurosecretory hormone required for egg development in mosquito. Gen. Comp. Endocrinol., 16, 105, 1971.
3. Adams, T.S., Grugel, S., Ittycherian, P.I., Olstad, G., Caldwell, J.M., Interactions of the ring gland, ovaries, and juve nile hormone with brain neurosecretory cells in Musca domestica. J. In sect Physiol., 21, 1027, 1975.
4. Ulrich, G.M., Schlagintwelt, B., Eder, J., Rembold, H., Elimination of the allatotrophic activity in locust by microsurgical and immunological methods: Evidence for humoral control of the corpora allata, haemolymph proteins, and ovary development, Gen. Comp. Endo crinol., 59, 120, 1985.
5. Toyoda, I., Numata, H., Sakiko, S., Role of the median neurosecretory cells in the ovarian development of the blow fly Protophormia terraenovae, Zool. Sci. 16(2), 187, 1999.
6. Adams, T.S., The ovaries, ring gland, after neurosecretion dur ing the second gonotrophic cycle in the housefly, Musca domestica, Gen. Comp. Endocrinol., 30, 69, 1976.
7. Ejezie, G.C., Davey, K.G., Changes in the neurosecretory cells, corpus cardiacum, and corpus allatum during pregnancy in Glossina austeni, Bull. Entomol. Res., 64, 247, 1974.
8. Juberthie, C., Caussanel, C., Release of brain neurosecretory products from the neurohaemal part of the aorta during egg-laying and egg-care in Labidura riparia, (Insecta: Dermaptera), J. Insect Physiol., 26, 427, 1980.
9. Siew, Y.S., The endocrine control of adult reproductive diapause in the chrysomelid beetle, Galeruca tanaceti (L.), III, J. Insect Physiol., 2, 973, 1965.
10. Ewen, A.B., An improved aldehyde fuchsin staining technique for neurosecretory products in insects, Trans. Am. Micr. Soc., 81, 94, 1962.
11. Park, Y.I., Ramaswamy, S.B., Role of brain, Ventral nerve cord, and corpora cardiaca-corpora allata complex in the reproductive behavior of female Tobacco budworm (Lepidoptera: Noctuidae), Ann. Entomol. Soc. Am., 91(3), 329, 1998.
12. Rohdendorf, E.B., Watson, J.A.L., The control of reproductive cycles in the female firebrat, Lepismodes inquilinus, J. Insect Physiol., 1, 2085, 1969.
13. Leob, M.J., Hayes, D.K., Neurosecretion during dia pause and diapause development in brains of mature embryos of the gyp sy moth, Laymantria dispar, Ann. Entomol. Soc. Am., 73, 432, 1980.
14. Khan, M.A., Romberg-Privee, H.M. and Schooneveld, H., Innervation of the corpus allatum in the Colorado Potato Beetle as revealed by retrograde diffusion with horseradish peroxidase, Gen. Comp. Endocrinol., 55, 66, 1984.
15. King, R.C., Aggarwal, S.K., Bodenstein, D., The comparative submicroscopic cytology of the corpus allatum-corpus cardiacum complex of wild type and fes adult female Drosophila melanogaster, J. Exp. Zool., 161(2), 151, 2005.
16. Mc Caffery, A.R., Effect of electrocoagulation of cerebral neurosecretory cells and implantation of corpora allata on oocyte develop ment in Locusta migratoria. J. Insect Physiol., 22, 1081, 1976.
17. Pathak, S.C., Mukerji, R., The corpus allatum in relation to sexueal behavior andaggressiveness in male Supella supellectilium. J. Insect. Physiol., 35(10), 751, 1989.
18. Paulson, G.E., Stay, B., Humoral inhimition of the corpora allata in larveae of Diploptera punctata: Role of the brain and ecdysteroids, J. Insect Physiol., 33(9), 613, 1987.
19. Readio, J., Peck, K., Meola, R., Dahm, K.H., Corpus allatum activity (in vitro) in female Culex pipiens during adult life cycle, J. Insect Physiol., 34(2), 131, 1988.
20. Stay, B., Tobe, S.S., Control of the corpora allata during a reproductive cycle in a viviparous cockroach. Amer. Zool., 21, 663, 1981.
21. Tombes, A. and Smith, D.S., Ultrastructural studies on the corpora cardiaca-allata complex of the adult alfalfa weevil, Hypera postica, J. Morphol., 132(2), 137, 2005.
22. Özlük, A., The cerebral neurosecretory system an the diversi ty of the neurosecretory ceil types in Pimpla turionellae L. (Hymenoptera: Ichneumonidae). Commun. Fac. Sic. Univ. Ank. Ser: C., 9, 33, 1991.
23. Pratt, G.E., Davey, K.G., The corpus allatum and oo genesis in Rhodnius prolixus (STAL.) I. The effects of allatectomy, J. Exp. Biol., 56, 215, 1972.
24. Briegel, H., Metabolic relationship between female body size, reserves and fecundity of Aedes aegypti. J. insect Physiol., 36(3), 165, 1990.
25. De Wilde, J., Reproduction-Endocrine Control. In the Physiol ogy of Insecta (Ed. by. Rockstein, M). 1, pp. 59-88, 1964.
26. Engelmann, F., Endocrine control of reproduction in insects. Ann. Rev. Entomol., 13, 1, 1968.
27. Panov, A.A., Demonstration of neurosecretory cells in the insect central nervous system. In “Neuroanatomical Techniques” Ed by NJ Strausfeld, TA Miller, Springer- Verlag, New York, pp. 25-50, 1980.
28. Pelletier, J.D., Turcotte, D.L., Osorio, S., Piulachs, M.D., Belles, X, Feeding and activation of corpora allata in the cockroach Blattella germanica (L.) (Dictyoptera: Blattellidae), J. Insect Physiol., 44(1), 31, 1997.
29. Bonetti A.M., Siquieroli, A.C., Santana, F.A., Vieira, C.U., Bezerra, M., Pereira, G.B., Simões, Z.L., Cruz-Landim, C., Effect of juvenile hormone III on the ultrastructure of the corpora allata in Melipona quadrifasciata (Hymenoptera, Apidae, Meliponını). Braz. J. Morphol. Sci., 23(3-4), 393, 2006.
30. Gilbert, I.L., Dia, J., An ultrastructural and developmental analysis of the corpus allatum of juvenile hormone deficient mutants of Drosophila melanogaster. Roux’s Arch. Dev. Biol., 202, 85, 1993.
31. Greenplate, J.T., Glaser, R.L., Hagedorn, H.H., The role of factors from the head in the regulation of egg development in the mosquito Aedes aegypti. J.Insect Physiol., 31, 325, 1985.
32. Huerta, C., Martinez, M.I., Morphological changes in reproductive organs and neuroendocrine centers related to nesting, mating, and larvicide behavior in Eurysternus mexicanus harold (Scarabaeinae: Eurysternini) The Coleopterists Bulletin 62(1), 123, 2008.
33. Bhaskaran, G., Jones, G., Neuroendocrine regulation of corpus allatum activity in Manduca sexta. The endocrine basis for star vation-induced supernumerary larval moult. J. Insect Physiol., 26, 431, 1980.
34. Bell, W.J., Barth, R.H., Initiation of yolk deposition by juvenile hormone. Nature, New Biol., 230, 220, 1971.
35. Mwangi, R.W., Goldsworthy, G.J, The effects of allatectomy and ovariectomy on haemolymph proteins and lipid metabolization in Locusta.J. Insect Physiol., 26, 741,1980.
36. Pratt, G.E., Davey, K.G., The corpus allatum and oo genesis in Rhodnius prolixus (STAL.) III. The effect of mating. J. Exp. Biol., 56, 223, 1972.
37. Tabakoğlu, A., Neurosecretory cell types and “C - cysteine incorporation in adult female Calliphora vicina. ROB. - DESV. (ERYTH-ROCEPHALA MEIG.) Istanbul Univ. Fen Fak. Biyoloji Der., 53, 45, 1988.
38. Adams, T.S., Hintz, M.M., Pomonis, J.G., Oostatic Hormone Production in Houseflies, Musca domestica, with Developing Ovaries, J. Insect Physiol., 14, 983, 1968.
39. Sefiani, M., Regulation of Egg Laying and in Vitro Oviducal Contractions in Gryllus bimaculatus, J. Insect Physiol., 3(4), 215,1987.