Murat ŞENTÜRK, Müslüm KUZU, Mehmet ÇİFTÇİ

The effect of safranin on glutathione reductase and glucose 6-phosphate dehydrogenase enzymes

Bu çalışmanın amacı safraninin insan eritrosit glutayon redüktaz (GR) ve glukoz 6-fosfat dehidrogenaz (G6PD) üzerindeki inhibitör etkilerini değerlendirmektir. Bu amaçla, 2’,5’-ADP Sepharose 4B afinite jeli ve Sephadex G-200 jel filtrasyon kromatografisi kullanılarak insan eritrosit glutayon redüktaz 2175-kat (%31.35 verim) ve 2’,5’-ADP Sepharose 4B afinite jeli kullanılarak eritrosit glukoz 6-fosfat dehidrogenaz 8681.3-kat (%24.77 verim) ile saflaştırıldı. Safranin in vitro GR ve G6PD inhibitör etkisi gösterdi. Safraninde IC50 değerleri, sırası ile GR için 1.15 mM ve G6PD için 3.22 mM, ve Ki sabitleri sırası ile 2.63 ± 0.13 ve 4.53 ± 0.17 mM oldu. Safranin GR ve G6PD için yarışmasız inhibisyon gösterdi.

Glutatyon redütaz ve glukoz 6-fosfat dehidrogenaz enzimleri üzerine safraninin etkisi

The aim of this study was to assess inhibitory effect of safranin on human erythrocyte glutathione reductase (GR) and glucose 6-phosphate dehydrogenase (G6PD). For this purpose, human erythrocyte glutathione reductase was purified 2175-fold (31.35% yield) using 2’,5’-ADP Sepharose 4B affinity gel and Sephadex G-200 gel filtration chromatography and erythrocyte glucose 6-phosphate dehydrogenase was purified 8681.3-fold (24.77% yield) using 2’,5’-ADP Sepharose 4B affinity gel. Safranin exhibited inhibitory effects on GR and G6PD enzymes in vitro. The IC50 values of safranin were 1.15 mM for GR and 3.22 mM for G6PD, respectively, and the Ki constants were 2.63 ± 0.13 and 4.53 ± 0.17 mM, respectively. Safranin displayed noncompetitive inhibition for GR and G6PD.

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