Polianilin-Poli 3-metiltiyofen -Poli 3,3’-diaminobenzidin Filmi ile Modifiye Edilmiş Pt Elektrot ile Nehir Suyunda Pirokatekolün Elektroanalizi
N aHSO4/Na2SO4 SBS pH 2.0 çözeltisi içerisinde amperometrik I-t yöntemi kullanılarak polianilin-poli 3metiltiyofen -poli 3,3’-diaminobenzidin PANI - P3MT - PDAB kaplı Pt disk elektrot üzerinden pirokatekol tayini başarılı bir şekilde gerçekleştirildi. 0,45 V–0,65 V aralığında potansiyel değerleri uygulandı ve 0.50 V optimum potansiyel olarak belirlendi. Analitik parametreler gözlenebilme sınırı, alt tayin sınırı ve doğrusal çalışma aralığı sırasıyla 7.37×10-5, 2.47×10-4 ve 2.47×10-4-25.0 mmol.L-1 olarak bulundu. Ayrıca, matriks girişimini ve geliştirilen yöntemin doğruluğunu test etmek için, pirokatekol derişimi 5.0 ve 10.0 mmol.L-1 olacak şekilde yapay olarak kirletilmiş nehir sularında pirokatakol tayini gerçekleştirildi. Geri kazanım değerleri sırasıyla % 101.1 ve % 101.2, olarak elde edildi. Pirokatekolün yükseltgenme potansiyelinden 0.62 V daha düşük potansiyelde 0.50 V matriks etkisi olmaksızın pirokatakol tayini gerçekleştirildi
Electroanalysis of Pyrocatechol in River Water Over Pt Electrode Modified with Polyaniline-Poly 3-methylthiophene - Poly 3,3’-diaminobenzidine Film
Determination of pyrocatechol was successfully achieved over polyaniline - poly 3-methylthiophene - poly 3,3’-diaminobenzidine PANI - P3MT - PDAB coated Pt disc electrode using amperometric I-t method in NaHSO4/Na2SO4 SBS pH 2.0 solution. The potential range of 0.45 V-0.65 V were implemented and a potential of 0.50 V was found to be an optimum potential. Analytical parameters LOD, LOQ and linear range were 7.37×10-5, 2.47×10-4 and 2.47×10-4-25.0 mmol.L-1, respectively. Furthermore, assay of pyrocatechol was performed in artificially contaminated river water samples over modified electrode at the pyrocatechol concentrations of 5.0 and 10.0 mmol.L-1 to check on the matrices interference and accuracy of the developed method. Recovery values were 101.1% and 101.2%, respectively. Without matrix effect, determination of pyro- catechol at lower peak potential 0.50 V than oxidation peak potential 0.62 V was carried out.
___
- N. Alizadeh, H. Zarabadipour, A. Mohammadi, M.R. Nateghi, M.H. Mosslemin H. Hadjimohammadi, Headspace solid-phase microextraction using an electrochemically deposited dodecylsulfate-doped polypyrrole film to determine of phenolic compounds in water, Anal. Chim. Acta, 605 (2007) 159.
- Q. Ameer, S.B. Adeloju, Development of a potentiometric catechol biosensor by entrapment of tyrosinase within polypyrrole film, Sens. Actuators B: Chem., 140 (2009) 5.
- M. Castillo, R. Domingues, M.F. Alpendurada D. Barceló, Persistence of selected pesticides and their phenolic transformation products in natural waters using off-line liquid solid extraction followed by liquid chromatographic techniques, Anal. Chim. Acta, 353 (1997) 133.
- R. Gatti, M.G. Gioia, A.M. Di Pietra V. Cavrini, Analysis of phenols in pharmaceuticals by liquid chromatography after pre-column labelling and on- line post-column photochemical derivatization, Anal. Chim. Acta, 447 (2001) 89. determination of phenols in water, Anal. Chem., 47 (1975) 1325. phenolics in food, J. Chromatogr. A, 1054 (2004) 95.
- G. Wang, X. He, F. Zhou, Z. Li, B. Fang, X. Zhang L. Wang, Amperometric determination of hydroquinone and catechol on gold electrode modified by direct electrodeposition of poly(3,4-ethylene dioxythiophene), Sens. Actuators B: Chem., 193 (2014) 212.
- M. Kavanoz N.Ö. Pekmez, Poly(vinylferrocenium) perchlorate–polyaniline composite film-coated electrode for amperometric determination of hydroquinone, J. Solid State Electrochem., 16 (2012)
- Y. Tan, X. Guo, J. Zhang J. Kan, Amperometric catechol biosensor based on polyaniline–polyphenol oxidase, Biosens. Bioelectron., 25 (2010) 1681.
- H. Zhang, A. Galal, J.F. Rubinson, I. Marawi, T.H. Ridgway, S.K. Lunsford, H. Zimmer H.B. Mark Jr, Flow injection amperometric detection of catechol using dual-band poly(3-methylthiophene) electrodes, Electrochim. Acta, 43 (1998) 3511.
- M. Kavanoz, U. Bük E. Ülker, Determination of hydroquinone using poly(3-methylthiophene) synthesized electrochemically on Pt electrode in methylene chloride, J. Appl. Polym. Sci., 131 (2014).
- Q. Cai S.B. Khoo, Poly(3,3’-diaminobenzidine) film on a gold electrode for selective preconcentration and stripping analysis of selenium(IV), Anal. Chem., 6 (1994), 4543.
- I.E. Mülazımoğlu, A.D. Mülazımoğlu E. Yılmaz, Determination of quantitative phenol in tap water samples as electrochemical using ,3’-diaminobenzidine modified glassy carbon sensor electrode, Desalination, 268 (2011) 227.
- Electrochemical preparation and characterization of poly(3,3-diaminobenzidine): A functionalized polymer, React. Funct. Polym., 64 (2005) 103.
- E. Ulker M. Kavanoz, Synthesis of poly(vinylferrocene) perchlorate/poly(3,3’-Diaminobenzidine) modified electrode in dichloromethane for electroanalysis of hydroquinone, J. Braz. Chem. Soc., 26 (2015) 1947.
- M. Kavanoz, E. Ülker U. Bük, A novel polyaniline–Poly(3- methylthiophene)–poly(3, 3-diaminobenzidine) electrode for the determination of dopamine in human serum, Anal. Lett., 48 (2015) 75.
- E. Ulker M. Kavanoz, Determination of epinephrine in human serum over polyaniline-poly(3- methylthiophene)-poly(3, 3’-diaminobenzidine) modified Pt electrode, Can. J. Chem., 93 (2015) 1239.
- G.L. Long J.D. Winefordner, Limit of detection a closer look at the IUPAC definition, Anal. Chem., 55 (1983) 712.
- P. Yang, Q. Zhu, Y. Chen F. Wang, Simultaneous determination of hydroquinone and catechol using poly(p-aminobenzoic acid) modified glassy carbon electrode, J. Appl. Polym. Sci., 113 (2009) 2881.
- Y. Umasankar, A.P. Periasamy S.-M. Chen, Electrocatalysis and simultaneous determination of catechol and quinol by poly(malachite green) coated multiwalled carbon nanotube film, Anal. Biochem., 411 (2011) 71.
- Y. Kong, Y. Xu, H. Mao, C. Yao X. Ding, Expanded graphite modified with intercalated montmorillonite for the electrochemical determination of catechol, J. Electroanal. Chem., 669 (2012) 1.
- L. Wang, P. Huang, J. Bai, H. Wang, L. Zhang Y. Zhao, Simultaneous electrochemical determination of phenol isomers in binary mixtures at a poly(phenylalanine) modified glassy carbon electrode, Int. J. Electrochem. Sci., 1 (2006) 403.
- S. Campuzano, B. Serra, M. Pedrero, F.J.M.D. Villena J.M. Pingarrón, Amperometric flow-injection determination of phenolic compounds at self- assembled monolayer-based tyrosinase biosensors, Anal. Chim. Acta, 494 (2003) 187.