Erkay ÖZGÖR, Meltem ULUSOY, İrem ÇELEBİER, Semiha Selda YILDIZ, Nevin KESKİN

Farklı Trametes versicolor Özütlerinin Bazı Klinik İzolatlar Üzerindeki Antimikrobiyal Aktivitesinin İncelenmesi

K linik önemi olan ve ciddi hastalıklara sebep olan türlere karşı yoğun ilaç kullanımı bu türlerde direnç gelişimini tetiklemektedir. İlerleyen yıllarda birçok antimikrobiyal ilaç etkisiz hale geleceğinden bu türlere karşı yeni potansiyel ilaçların keşfedilmesi gereklidir. Bu araştırmada, Trametes versicolor üreme yapılarından elde edilen farklı özütler, bazı klinik izolatlar üzerinde antibakteriyel ve antifungal aktiviteleri araştırmak için kullanılmıştır. Bu amaç doğrultusunda, klinik önemi olan izolatlar olarak 5 bakteri türü Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa ile bir fungus türü Candida albicans seçildi ve Trametes versicolor’un 4 farklı ham özütünün kloroform, su, etil asetat, etil alkol , bu türler üzerinde antimikrobiyal duyarlılık açısından disk difüzyon yöntemi kullanılarak denendi. Elde edilen veriler SPSS 14 programı kullanılarak analiz edildi. Çalışmanın sonunda, tüm özütler genel olarak antimikrobiyal etki gösterse de özütlerden bazılarının klinik izolatlar üzerinde antimikrobiyal aktiviteye sahip olduğu bulunmuştur. Kloroform özütünün S. aureus ve E. faecalis üzerinde en yüksek antimikrobiyal aktiviteye sahip olduğu bulunurken etanol özütünün P. aeruginosa, E. coli, B. subtilis ve C. albicans izolatları üzerinde daha etkili olduğu gözlenmiştir. Bu nedenle, bazı Trametes versicolor özütlerinin umut verici antimikrobiyal ajanlar olabileceği söylenebilir

Anahtar Kelimeler:

Trametes versicolor, Antimikrobiyal aktivite, Antifungal aktivite, Klinik izolatlar

Investigation of Antimicrobial Activity of Different Trametes versicolor Extracts on Some Clinical Isolates

Intensive drug uses against species which have clinical significance and cause severe diseases have triggered the development of resistance in these species. It is necessary to discover new potential drugs against cli- nical isolates because many antimicrobial drugs become ineffective in the coming years. In this research, we used different extracts from Trametes versicolor fruiting bodies for investigating antibacterial and antifungal activities on some clinical isolates. For this purpose, five bacterial species Staphylococcus aureus, Entero- coccus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa and a fungal species Candida albicans were selected as clinically important isolates and four different crude extracts chloroform, water, ethyl acetate and ethyl alcohol of Trametes versicolor were tried on these species in terms of antimicrobial sensitivity with disc diffusion method. Obtained data were analyzed using SPSS 14 software. At the result, some of extracts were found to have antimicrobial activity on clinical isolates although all extracts generally show antimicrobial effect. Chloroform extract was found to have the highest antimicrobial activity on S. aureus and E. faecalis while ethanol extract was observed as more effective on P. aeruginosa, E. coli, B. subtilis and C. albicans isolates. It can therefore be suggested that, some of Trametes versicolor extracts are promising antimicrobial agents.

Keywords:

Trametes versicolor, antimicrobial activity, antifungal activity, Clinical isolates,

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