Sibel SÜMER, Sibel ÜNLÜ

PCR based relp analysis of an intergenic spacer region in cpDNA of some AEGILOPS and Triticum species

Restriction fragment length polymorphism analysis of some Aegilops and Triticum species were performed in this study. An intergenic spacer region of chloroplast DNA (cpDNA) was digested by five different restriction endonuclease enzymes. Obtained fragments were investigated on agarose gel electrophoresis technique. Phylogenetic relationships among Triticum and Aegilops species were discussed, based on the resultant data.

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1. Doebley, J., Durbin, M., Golenberg, E.M., Clegg, M.T. and Ma, D.P. Evolutionary analysis of the large subunit of carboxylase (rbcL) nucleotide sequence among the grasses (Gramineae). Evolution, 44(4), 1097-1108,1990.
2. Briggle, L.W. and Reitz, L.P. Classification of Triticum species and of wheat varieties grown in the United States. U.S. Dept. Of Agriculture Bulletin, 1278,1963.
3. Simmonds, N.W. Evolution of Crop Plants. Longman, London, p49, 1976.
4. Golenberg, E.M., Clegg, M.T., Durbin, M.L., Doebley, J. Ma, D.P. Evolution of a noncoding region of the chloroplast genome. Molecular Phylogenetics and Evolution, 2, 52-64,1993.
5. Clegg, M.T., Gaut, B.S., Learn Jr., G.H. and Morton, B. Rates and patterns of chloroplast DNA evolution. PNAS USA., 6795-6801,1994.
6. Morton, B.R. Neighbouring base composition and transversion/transition bias in a comparison of rice and maize chloroplast noncoding regions. PNAS USA., 92, 9717-9721,1995.
7. Clegg, M.T., Cummings, M.P. and Durbin, M. The evolution of plant nuclear genes. PNAS USA., 94,7791-7798,1997.
8. Morton, B.R. Strand asymmetry and codon bias in the chloroplast genome of Euglena gracilis. PNAS USA., 96, 5123-5128,1999.
9. Stoebe, B. and Kowallik, K.L. Gene-cluster analysis in chloroplast genomics. TIG., 15, 344-347,1999.
10. Clegg, M.T. Chloroplast gene sequences and the study of plant evolution. PNAS USA.,90,363-367,1993.
11. Howe, C, Barker, R.F., Bowman, CM. and Dyer, T.A. Common features of three inversions in wheat chloroplast DNA. Curr. Genet., 13, 343-349, 1988.
12. Hiratsuka, J., Shimada, H., Whittier, R., Ishibashi, T., Sakamoto, M., Mori, M., Kondo, C, Honji, Y., Sun, CR., Meng, B.Y., Li, Y.Q., Kanno, A., Nishizawa, Y., Hirai, A., Shinozaki, K. and Sugiura, M. The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol. Gen. Genet., 217, 185-194,1989.
13. Ogihara, Y. and Tsunewaki, K. Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis. Theor. Appl. Genet. , 76. 321-332, 1988.
14. Palmer, J.D. Plastid chromosomes: structure and evolution. In: (Eds.) L Bogorad, I.K. Vasil. The molecular biology of plastids. San Diego: Academic Press, 55-53, 1991.
15. Kung, S.S., Zhu, Y.S. and Shen, G.F. Nicotiana chloroplast genome. III. Chloroplast DNA evolution. Theor. Appl. Genet., 61, 73-79,1982.
16. Gordon, K.H.J., Crouse, E.J. Bohnert, H.J. and Herman, R.G. Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera. Theor. Appl. Genet., 61,373-384, 1982.
17. Palmer, J.D. Comparative organization of chloroplast genomes. Ann. Rev. Genet., 19, 325-354,1985.
18. Morton, B.R. and Clegg, M.T. A chloroplast DNA mutational hotspot and gene conversion in a noncoding region near rbcL in the grass family (Poacea). Current Genetics, 24: 357-365, 1993.
19. Doyle, J.T. and Doyle, J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19(1), 11-15, 1987.
20. Buntjer JB. Cross Checker V2.91. Department of Plant Breeding, Wageningen University and Research Center, The Netherlands, 1999.
21. Rohlf FJ. NTSYSpc V2.10. Applied Biostatistics Inc., Exeter Software-New York, 2000.
22. Ogihara, Y., Terachi, T., Sasakuma, T. Molecular analysis of the hot spot region related to length mutations in wheat chloroplast DNAs. I. Nucleotide divergence of genes and intergenic spacer regions located in the hot spot region. Genetics, 129, 873-884, 1991.
23. Kihara, H. Discovery of the DD-analyser, one of the ancestors of Vulgare wheats. Agric. Hort. (Tokyo), 19, 13-14,1944.
24. McFadden, E.S., Sears, E.R. The origin of Triticum spelta and its freethreshing hexaploid relatives. J. Hered., 37, 81-89, 107-116, 1946.
25. Kovacs,G., Paldi, E., Racz, I., Lasztity, D. Nucleotide Sequence of Chloroplast 16S rDNA (Accession No: AJ239003) from Hexaploid wheat Triticum aestivum L. (PGR00- 011) Plant Physiol., 122, .292,2000.
26. Bowman, CM., Bonnard, G., Dyer, T.A. Chloroplast DNA variation between species of Triticum and Aegilops: location of the variation on the chloroplast genome and its relevance to the inheritance and classification of the cytoplasm. Theoretical and Applied Genetics, 65,247-262, 1983.
27. Timothy, D.H., Levings, C.S., III, Pring, DR., Conde, M.F. and Kermicle, J.L. Organelle DNA variation and systematic relationships in the genus Zea: Teosinte. PNAS, USA, 6,4220-4224, 1979.
28. Tsunewaki, K., Ogihara, Y. The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. II. On the origin of polyploidy wheat cytoplasms as suggested by chloroplast DNA restriction fragment patterns. Genetics, 104, 155-171, 1983.
29. Palmer, J.D., Jorgensen, R.A. Thompson WF. Chloroplast DNA variation and evolution in pisum: Patterns of change and phylogenetic analysis. Genetics, 109, 195-213, 1985.
30. Huang, S., Sirikhachornkit, A., Su, X., Fan's, J., Gill, B., Haselkorn, R., Gornicki, P. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the TriticumlAegilops complex and the evolutionary history of polyploid wheat. PNAS, USA, 99( 12), 8133-8138,2002.