Tuğba TAŞKIN TOK, Nuriye AKBAY, Zeynep KÖKSAL, Ayşe UZGOREN BARAN

Naproksen Türevi ile ct-DNA’nın Etkileşim Özellikleri

Naproksenin bir türevi (NH) ile ct-DNA’nın etkileşim türü absorpsiyon ve floresans spektroskopisi ile incelendi. Deneysel sonuçlar, naproksen türevi ile ct-DNA arasında oluğa bağlanma sonucunda statik sönüm gerçekleştiğini ortaya koymuştur. Hesaplamalı yöntemler de etkileşimin daha detaylı anlaşılması için kullanılmıştır. Moleküler kenetlenme hesaplamaları, NH ve ct-DNA arasındaki etkileşimin oluğa tutunma üzerinden olduğunu göstermiştir. Spektral verilere ek olarak, kenetlenme çalışmaları NH-A_DNA ve NH-B_DNA komplekslerinin her bir DNA izomerine özgü farklı etkileşim ve konformasyon eğilimlerine sahip olduğunu göstermiştir.

Naproxen Derivative Interaction Properties with ct-DNA

Interaction mode of a naproxen derivative (NH) with ct-DNA was explored by absorption and fluorescence spectroscopy.The experimental results revealed the static quenching as a result of groove binding between the naproxen derivativeand ct-DNA. Computational studies were carried out to a deeper understanding of the interactions. Molecular dockingcalculations showed that the interaction between NH and ct-DNA is resulted by groove binding. In addition to spectral data,docking studies revealed that NH-A_DNA and NH-B_DNA complexes had different interaction and conformational trendsto each DNA isomer.

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