Aysun KILIÇ, Cansın GÜNGÖRMÜŞ, E. Arzu KOÇKAYA, Dürdane KOLANKAYA, M. Turan AKAY

Gıda Katkı Maddesi Olan Trikalsiyum Fosfatın Gebe Sıçanlar ve Fötusları Üzerinde Toksik Etkisi Var mıdır?

B u çalışmanın amacı bir gıda katkı maddesi olan Trikalsiyum fosfat TCP ’ın Wistar sıçanlarda maternal ve fötal toksisitesini değerlendirmektir. 0-20. gebelik günlerinde GD TCP’nin E341 iki dozu olan 175 mg/kg vücut ağırlığı/gün TCP I grubu ve 350 mg/kg vücut ağırlığı/gün TCP II grubu . TCP maruziyetinin olası toksik etkileri biyokimyasal, histopatolojik ve morfolojik analizler ile araştırılmıştır. Bütün gebe sıçanlar maternal vücut ağırlığı, yem ve su tüketimi açıdan incelenmiş ve GD 20’de sezeryan yapılmıştır. Maternal hemotolojik analizlere göre, lenfosit, monosit ve nötrofil yüzdeleri TCPI ve TCPII gruplarında değişmiştir. Biyokimyasal analizler aspartat aminotransferaz, alanin aminotransferaz enzim aktiviteleri ve üre, kreatin seviyelerinde değişiklik olmadığını; TCPI grubunda alkalin fosfataz aktivitesinin arttığını göstermektedir. TCP uygulama gruplarında maternal karaciğer, böbrek, kalp, beyin ve fötal karaciğer, böbrek ve plasenta dokularında histopatolojik değişiklikler tespit edilmiştir

Anahtar Kelimeler:

TCP, histopatoloji, plasenta, E341

Are There any Toxic Effects of Food Additive Tricalcium Phosphate on Pregnant Rats and Their Fetuses?

The aim of the study was to evaluate the maternal and fetal developmental toxicity of food additive Tricalcium phosphate TCP in Wistar rats. Two doses of TCP E341 175 mg/kg bw/day TCP I group and 350 mg/kg bw/day TCP II group were administered to rats during gestation days GD 0-20. The possible toxic effects of TCP exposure were investigated by biochemical, histopathological and morphological examinations. All dams were observed for maternal body weights, food and water consumptions and subjected to caesarean- section on gestation day GD20. According to maternal haematological analysis, lymphocyte, monocyte and neutrophil percentages were changed in TCP I and TCP II groups. Biochemical analysis showed that while enzyme activi- ties of aspartate aminotransferase, alanine aminotransferase and levels of urea and creatinine did not change among groups; alkaline phosphatase activity increased in TCP I group. There were histopathological changes in maternal liver, kidney, heart, brain tissues and fetal liver, kidney and placenta tissues in treatment groups.

Keywords:

TCP, histopathology, placenta, E341,

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