Sezen BİLEN ÖZYÜREK, Sinem DİKEN GÜR, Işıl Seyis BİLKAY

Farklı Klinik Malzemelerden İzole Edilen Pseudomonas aeruginosa Suşlarıyla Üretilen Piyosiyaninin Antimikrobiyal Etkisinin Araştırılması

P seudomonas aeruginosa suşları tarafından üretilen piyosiyanin karakteristik mavi-yeşil bir fenazin pigmentidir. Çalışmanın amacı, P. aeruginosa suşlarında en yüksek piyosiyanin üretiminin belirlenmesi ve piyosiyaninin Escherichia coli, Bacillus sp., Pseudomonas aeruginosa, Candida sp. and Aspergillus niger üzerindeki antimiktobiyal etkisinin araştırılmasıdır. Bu doğrultuda, piyosiyanin P. aeruginosa kültür süpernatanından özütlendi ve saflaştırıldı, ardından spektrofotometrik olarak ölçüldü. En yüksek piyosiyanin üretiminin idrar örneğinden izole edilen P. aeruginosa A10 suşunda ve en düşük piyosiyanin üretiminin batından izole edilen P. aeruginosa A1 suşunda olduğu belirlendi. Piyosiyaninin antimikrobiyal etkisinin belirlenmesinde ‘Agar Kuyu Difüzyon’ yöntemi uygulandı. Böylece, piyosiyanin Bacillus sp., Escherichia coli ve Candida sp., üzerinde etkili bir antimikrobiyal ajanken, P. aeruginosa ve A. niger üzerinde antimikrobiyal etkisi görülmedi. Ardından, agar kuyu yöntemi ile piyosiyanine duyarlı olduğu belirlenen suşlara ‘Tüp Seyreltme Yöntemi’ kullanıldı. Azalan piyosiyanin derişimleri ile Bacillus sp. and Candida sp. suşlarının üremesinde artış gözlendi

Anahtar Kelimeler:

Pseudomonas aeruginosa, Piyosiyanin, Antimikrobiyal Aktivite.

Investigation of Antimicrobial Activity of Pyocyanin Produced by Pseudomonas aeruginosa Strains Isolated from Different Clinical Specimens

Pyocyanin is the characteristic blue-green phenazine pigment produced by Pseudomonas aeruginosa stra- ins. The aim of our study is to determine the highest amount of pyocyanin production in P. aeruginosa strains and to investigate the antimicrobial effect of pyocyanin pigment on Escherichia coli, Bacillus sp., Pseu- domonas aeruginosa, Candida sp. and Aspergillus niger. For this purpose, pyocyanin was extracted from P. ae- ruginosa culture supernatants and purified, and then it was measured by spectrophotometrically. P. aerugino- sa A10 strain isolated from urine specimen was determined as the highest amount of pyocyanin producer and P. aeruginosa A1 strain isolated from abdomen was the lowest. The ‘Agar Well Diffusion’ method was carried out to determine the antimicrobial effect of pyocyanin. Accordingly, pyocyanin was effective as an antimicrobial agent on Bacillus sp., Escherichia coli and Candida sp., but there was no antimicrobial effect on P. aeruginosa and A. niger. After that ‘Tube Dilution Method’ was used on strains, which were determined as sensitive to pyocyanin with agar well diffusion method. An increase in the intensity of Bacillus sp. and Candida sp. strains’ growth with decreasing the concentration of pyocyanin was observed.

Keywords:

Pseudomonas aeruginosa, Pyocyanin, antimicrobial activity,

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