Geometrid Bir Güve olan Cyclophora Puppilaria (Lepidoptera)’nın Doğu Akdeniz Popülasyonlarındaki Allozim Lokuslardaki Genetik Varyasyon ve Mikroevrim
Genetic variation at 7 allozyme loci were determined in 4 Meditarranean populations of the moth Cyclophora puppillaria. Polymorphism levels, and gene diversities as Hardy-Weinberg heterozygosities are mostly substantial for the loci except for those in one of the samples. Pairwise genetic distances between the samples are expressed as $F_{ST}$ values, and a high degree of differentiation exists for almost all of the pairwise comparisons. A test of natural selection, Ewens-Watterson test of neutrality, shows no sign of selective difference among electrophoretic allelic states over loci in all samples. Our results presents the the first picture of population genetic variation in this species and in accordance with the result of a previous DNA barcoding analysis pointing to low level of genetic variation.
Genetic Variation and Microevolution at Allozyme Loci in Eastern Mediterranean Populations of the Geometrid Moth Cyclophora Puppillaria (Lepidoptera)
Genetic variation at 7 allozyme loci were determined in 4 Meditarranean populations of the moth Cyclophora puppillaria. Polymorphism levels, and gene diversities as Hardy-Weinberg heterozygosities are mostly substantial for the loci except for those in one of the samples. Pairwise genetic distances between the samples are expressed as $F_{ST}$ values, and a high degree of differentiation exists for almost all of the pairwise comparisons. A test of natural selection, Ewens-Watterson test of neutrality, shows no sign of selective difference among electrophoretic allelic states over loci in all samples. Our results presents the the first picture of population genetic variation in this species and in accordance with the result of a previous DNA barcoding analysis pointing to low level of genetic variation.
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- 1. B. Charlesworth, D. Charlesworth, Elements of Evolutionary Genetics. Roberts and Company Publishers, Greenwood Village Colorado (2010).
- 2. R.C. Lewontin, Genetic basis of Evolutionary Change. Columbia University Press, NewYork & London, (1974).
- 3. D.M. Hillis, C. Moritz, B.K. Mable, Molecular Systematics. Sunderland & Massachusetts: Sinauer Associates, (1996).
- 4. A. Barbadilla, L.M. King, R.C. Lewontin, What does electrophoretic variation tell us about protein variation? Molecular Biology and Evolution, 13 (1996) 427-432.
- 5. A. Hausmann, The Geometrid Moths of Europe. Volume 1. Apollo Books, Stenstrup Denmark, (2001).
- 6. K. Mazzucco, Cosymbia pupillaria Hbn. Bei 3200 m. Z. Wien. Ent. Ges. 51 (1966) 154.
- 7. A. Hausmann, Geometrid Moths of Europe. Volume 2. ApolloBooks, Stenstrup, Denmark, (2004).
- 8. G.P. Manchenko, Handbook of Detection of Enzymes on Electrophoretic Gels. CRC Press, Vladivostok, Russia, (2003).
- 9. W.J. Ewens, The sampling theory of selectively neutral alleles. Theoretical Population Biology, 3 (1972) 87–112.
- 10. G.A. Watterson, The homozygosity test of neutrality. Genetics, 88 (1978) 405–417.
- 11. M. Slatkin, An exact test for neutrality based on the Ewens sampling distribution. Genetical Research, 64 (1994) 71-74.
- 12. L. Excoffier, G. Laval, S. Schneider, Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1 (2005) 47-50.
- 13. B. Weir, Genetic Data Analysis. Sinauer Association, Sunderland, MA, USA, (1990).
- 14. A. Hausmann, H.C.J. Godfray, P. Huemer, M. Mutanen, R. Rougerie, E.J. Nieukerken, S. Ratnasingham, Hebert,P.D.N., Genetic patterns in European Geometrid moths revealed by the barcode Index number (BIN) system. PLoS ONE 8e84518, 1-11 (2013).
- 15. J.A. Endler, Geographic Variation,Speciation, and Clines. Princeton University Press, Princeton (1977).