Çağatay KARAASLAN, Hayriye AKEL, Sibel ÜNLÜ, Işık PERÇİN

Buğdaydan DNA Ekstraksiyonu için Altı Ticari DNA Ekstraksiyon Kitinin Karşılaştırılması

B u çalışmada, 6 adet DNA ekstraksiyon kiti DNA verimi, DNA saflığı, amplifikasyon etkinliği, harcanan zaman ve ekstraksiyon başına düşen fiyat açısından karşılaştırılmıştır. Ekstrakte edilen DNA örnekleri, agaroz jel elektroforezi ve spektrofotometrik ölçümler ile kontrol edilmiştir. DNA örneklerinin PCR etkinliğini değerlendirmek için, ekstrakte edilen DNA örnekleri 18SrRNA kloroplast ve trnL–trnF genler arasındaki bölgenin amplifikasyonları için kalıp olarak kullanılmıştır. Sonuçlar, SIGMA GenElute™ Plant Genomic DNA Miniprep ve QIAGEN DNeasy Plant Mini kitlerinin buğday tohumundan ve yapraklarından DNA izolasyonunda en etkili ticari kitler olduğunu göstermiştir

Anahtar Kelimeler:

Buğday, DNA ekstraksiyon kitleri, DNA izolasyonu, Bitki DNA’sı

Comparison of Six Commercial DNA Extraction Kits for DNA Extraction from Wheat

In this study, six commercial DNA extraction kits were compared with each other in terms of DNA yield, DNA purity, amplification efficiency, the time spent and the cost per extraction. Extracted DNA samples were controlled by agarose gel electrophoresis and spectrophotometric measurement. The DNA samples were used as a template for 18SrRNA and chloroplast trnL–trnF intergenic spacer region amplifications to evaluate the PCR efficiency of DNA samples. Results showed that SIGMA GenEluteTM Plant Genomic DNA Miniprep Kit and QIAGEN DNeasy Plant Mini Kit are the most efficient commercial kits for DNA isolation from both wheat seed and leaf samples.

Keywords:

Wheat, DNA extraction kits, DNA isolation, Plant DNA,

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