Nesrin Ecem BAYRAM

Balda Polen Tiplerini Belirlemek için Moleküler Yaklaşım: DNA Barkodlama

Günümüzde doğal ürünlere olan ilginin artmasına bağlı olarak arı ürünlerine olan talep de artmıştır. Bu ürünlerdenbiri olan balın tağşişi oldukça kolay olmasına rağmen tağşişin tespit edilmesi de bir o kadar zordur. Sahteciliği tespitetme noktasında özellikle baldaki bitki kaynaklarını tespit etmeye yönelik çalışmalar yapılmaktadır. Balın botanik orijininitanımlamak için yaygın olarak kullanılan yöntemler, melissopalinolojik analiz ve kimyasal analizlerdir. Son yıllarda ise dahahızlı, basit ve güvenilir sonuç sunan DNA tabanlı yöntemler ile balın bitki kaynakları tespit edilmeye başlanmasına rağmenbu konuda yapılan çalışmalar Türkiye’de yok denecek kadar azdır. Bireysel polen tanelerinin görsel olarak incelenmesinigerektiren morfolojik tanımlama yöntemlerinin aksine, yakın zamanda geliştirilen genetik yaklaşımlar, polen analizlerininölçeğini ve çözünürlüğünü artırma potansiyeline sahiptir. Bu çalışmada balda moleküler teknikler kullanılarak yapılançalışmaların sonuçları derlenerek toplu bir veri sunulması amaçlanmış, yöntemin avantaj ve dezavantajları değerlendirilmiştir.

Molecular Approach to Detecting Pollen Types In Honey: DNA Barcoding

Nowadays, the demand for bee products has also increased due to the growing interest in natural products. Althoughit is quite easy to adulteration of honey, which is one of these products, it is also very difficult to detect adulteration.In the point of detecting forgery, studies are being carried out specifically to identify the plant sources of honey.Melissopalynological and chemical analyses are methods commonly used in order to identify the botanical origin of honey.Despite the fact that the detection of botanical origin of honey by DNA-based methods which provide faster, simpler andmore reliable results are being carried out in recent years, these researches are very less in Turkey. Unlike morphologicalmethods, which require the visual examination of pollen grains, the recently developed genetic methods have the potentialto increase the resolution and scale of pollen analyses. In this study, the aim was to present cumulative data by compilingthe results of the studies conducted using molecular techniques on honey, and the advantages and disadvantages of thistechnique were evaluated.

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