Yeliz DEMİR, Ayetullah TEMİZ, Ayşe ALBAYRAK, Yavuz ALBAYRAK, Şükrü BEYDEMİR, Mesut IŞIK, Hatice Esra DURAN, Adem ASLAN, Yılmaz ÖZDEMİR

Akut Apandisitlerde Hücresel Antioksidan Savunma Sisteminin Oksidatif Stres Üzerindeki Rolü

Serbest radikallerin, ürünlerinin ve reaktif oksijen türlerinin (ROS) birçok hastalıkla ilişkili olduğu bilinmektedir. Çalışmahem akut apandisitli (AA) hastalarda oksidatif stres faktörlerindeki hem de metabolizmada GST ve PON1 enzimaktivitelerindeki değişikliklere odaklanmaktadır. Bu çalışmaya kontrol grubu (n = 31) ve çalışma grubu (n = 52) dahil edildi.Gruplarda, metabolizmada önemli rol oynayan oksidatif stres faktörleri ve Glutatyon S-transferaz (GST) and paraoksanaz-I(PON1) enzim aktivitelerinde bazı değişiklikler belirlendi. Çalışma ve kontrol grupları arasında GST aktivitesinde önemlideğişiklikler belirlenmiştir. Bu enzim aktivitesi AA gruplarında (p

The role of the Cellular Antioxidant Defense System on Oxidative Stress in Acute Appendicitis

It is known that free radicals, their products and reactive oxygen species (ROS) are associated with many diseases. The study focuses on both some changes in oxidative stress factors in patients with acute appendicitis (AA) and the changes of glutathione S-transferase (GST) and paraoxonase-I (PON1) enzyme activities in the metabolism. This study involved control group (n = 31) and the study group (n = 52). In the groups, some changes in oxidative stress factors and GST and PON1 enzyme activities which play an important role in metabolism were determined. There are changes in GST activity between study and control groups. The activity of the enzyme were significantly higher in AA groups (p < 0.01) than control. Malondialdehyde (MDA) levels in acute perforated appendicitis (PeA) groups were found significantly higher than control (p

PDF

___

1. D.J. Shogilev, N. Duus, S.R. Odom, N.I. Shapiro, Diagnosing appendicitis: Evidence-based review of the diagnostic approach in 2014, West. J. Emerg. Med., 15 (2014) 859-871.
2. Y. Albayrak, A. Albayrak, M. Celik, I. Gelincik, I. Demiryilmaz, R. Yildirim, B. Ozogul, High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitis, Scand. J. Trauma. Resusc. Emerg. Med., 20 (2011) 19-27.
3. Y. Albayrak, A. Albayrak, F. Albayrak, R. Yildirim, B. Aylu, A. Uyanik, E. Kabalar, I.C. Güzel, Mean platelet volume: A new predictor in confirming acute appendicitis diagnosis, Clin. Appl. Thromb., 17 (2011) 362-366.
4. M.L. Nance, W.T. Adamson, H.L. Hedrick, Appendicitis in the young child: A continuing diagnostic challenge, Pediatr. Emerg. Care., 16 (2000) 160–162.
5. D.R. Flum, T. Koepsell, The clinical and economic correlates of misdiagnosed appendicitis: Nationwide analysis, Arch. Surg., 137 (2002) 799–804.
6. F.M. Yilmaz, G. Yilmaz, M.F. Erol, S. Köklü, D. Yücel, Nitric oxide, lipid peroxidation and total thiol levels in acute appendicitis, J. Clin. Lab. Anal., 24 (2010) 63–66.
7. H. SIES, Strategies of antioxidant defense, Eur. J. Biochem., 215 (1993) 213–219.
8. V. Stojiljković, A. Todorović, N. Radlović, S. Pejić, M. Mladenović, J. Kasapović, S.B. Pajović, Antioxidant enzymes, glutathione and lipid peroxidation in peripheral blood of children affected by coeliac disease, Ann. Clin. Biochem., 44 (2007) 537-543.
9. J.E. Klaunig, Y. Xu, J.S. Isenberg, S. Bachowski, K.L. Kolaja, J. Jiang, D.E. Stevenson, E.F. Walborg, The role of oxidative stress in chemical carcinogenesis, in: Environ. Health Perspect., 44 (1998) 239–267.
10. S.L. de Oliveira Machado, M.D. Bagatini, P. da Costa, J. Baldissarelli, K.P. Reichert, L.S. de Oliveira, J.G. Lemos, T. Duarte, M.R. Chitolina Schetinger,. V.M. Morsch, Evaluation of mediators of oxidative stress and inflammation in patients with acute appendicitis, Biomarkers., 21 (2016) 530-537.
11. M.S Özaslan, Y Demir, H.E Aslan, Ş Beydemir, Ö.İ Küfrevioğlu, Evaluation of chalcones as inhibitors of glutathione S-transferase, J. Biochem. Mol. Toxicol., 32(5) (2018) e22047.
12. Y. Demir, Naphthoquinones, benzoquinones, and anthraquinones: Molecular docking, ADME and inhibition studies on human serum paraoxonase-1 associated with cardiovascular diseases, Drug Dev. Res., 81 (2020) 628-636.
13. H. Paşaoǧlu, B. Sancak, N. Bukan, Lipid peroxidation and resistance to oxidation in patients with type 2 diabetes mellitus, Tohoku J. Exp. Med., 203 (2004) 211–218.
14. M. Kaya, M.E. Boleken, T. Kanmaz, O. Erel, S. Yucesan, Total antioxidant capacity in children with acute appendicitis, Eur. J. Pediatr. Surg., 16 (2006) 34–38
15. P.M. Reddy YN, Murthy S, Krishna DR, Role of free radicals and antioxidants in tuberculosis patients., Indian J Tuberc., 51 (2004) 213–218.
16. A.S. Veskoukis, M.G. Nikolaidis, A. Kyparos, D. Kokkinos, C. Nepka, S. Barbanis, D. Kouretas, Effects of xanthine oxidase inhibition on oxidative stress and swimming performance in rats, Appl. Physiol. Nutr. Metab., 33 (2008) 1140–1154.
17. M.S. Özaslan, Y. Demir, M. Aksoy, Ö.İ. Küfrevioğlu, Ş. Beydemir, Inhibition effects of pesticides on glutathione-S- transferase enzyme activity of Van Lake fish liver, J. Biochem. Mol. Toxicol., 32 (2018) e22196.
19. M. Gubandru, D. Margina, C. Tsitsimpikou, N. Goutzourelas, K. Tsarouhas, M. Ilie, A.M. Tsatsakis, D. Kouretas, Alzheimer’s disease treated patients showed different patterns for oxidative stress and inflammation markers, Food Chem. Toxicol., 61 (2013) 209-214.
20. F. Renault, E. Chabrière, J.P. Andrieu, B. Dublet, P. Masson, D. Rochu, Tandem purification of two HDL-associated partner proteins in human plasma, paraoxonase (PON1) and phosphate binding protein (HPBP) using hydroxyapatite chromatography, J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 836 (2006) 15–21.
21. M.L. Hu, Measurement of protein thiol groups and glutathione in plasma, Methods Enzymol., 233 (1994) 381– 385.
22. M.B. Grisham, Oxidants and free radicals in inflammatory bowel disease, Lancet., 344 (1994) 859–861.
23. T. Grune, M.M. Berger, Markers of oxidative stress in ICU clinical settings: Present and future, Curr. Opin. Clin. Nutr. Metab. Care., 10 (2007) 712-717.
24. R. Aktimur, A.K. Gokakin, K. Deveci, M. Atabey, O. Topcu, Oxidative stress markers in laparoscopic vs. open appendectomy for acute appendicitis: A double-blind randomized study, J. Minim. Access Surg., 12 (2016) 143-147.
25. J.N. Moloney, T.G. Cotter, ROS signalling in the biology of cancer, Semin. Cell Dev. Biol., 80 (2018) 50–64.
26. K. Yamamoto, P. Zhang, F. Miake, N. Kashige, Y. Aso, Y. Banno, H. Fujii, Cloning, expression and characterization of theta- class glutathione S-transferase from the silkworm, Bombyx mori, Comp. Biochem. Physiol. - B Biochem. Mol. Biol., 141 (2005) 340–346.
27. J.L. Hearne, R.F. Colman, Contribution of the mu loop to the structure and function of rat glutathione transferase M1-1, Protein Sci., 15 (2006) 1277–1289.
28. J. Han, E.J. Won, D.S. Hwang, J.S. Rhee, I.C. Kim, J.S. Lee, Effect of copper exposure on GST activity and on the expression of four GSTs under oxidative stress condition in the monogonont rotifer, Brachionus koreanus, Comp. Biochem. Physiol. - C Toxicol. Pharmacol., 158 (2013) 91–100.
29. M. Sohail, A. Kaul, M. Raziuddin, T. Adak, Decreased glutathione-S-transferase activity: Diagnostic and protective role in vivax malaria, Clin. Biochem., 40 (2007) 377–382.
30. M. Işık, Ş. Beydemir, A. Yılmaz, M.E. Naldan, H.E. Aslan, İ. Gülçin, Oxidative stress and mRNA expression of acetylcholinesterase in the leukocytes of ischemic patients, Biomed. Pharmacother., 87 (2017) 561-567.
31. Y. Demir, The behaviour of some antihypertension drugs on human serum paraoxonase-1: an important protector enzyme against atherosclerosis, J. Pharm. Pharmacol., 71 (2019) 1576-1583.
32. M. Işık, Ş. Beydemir, Y. Demir, M. Durgun, C. Türkeş, A. Nasır, A. Necip, M. Akkuş, Benzenesulfonamide derivatives containing imine and amine groups: Inhibition on human paraoxonase and molecular docking studies, Int. J. Biol. Macromol., 146 (2020) 1111-1123.
33. R. Esen, M. Aslan, M.E. Kucukoglu, A. Cıkman, U. Yakan, M. Sunnetcioglu, S. Selek, Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis, Wien. Klin. Wochenschr., 127 (2015) 427-433.
34. E.G. Dumlu, M. Tokaç, B. Bozkurt, M.B. Yildirim, M. Ergin, A. Yalçin, M. Kiliç, Correlation between the serum and tissue levels of oxidative stress markers and the extent of inflammation in acute appendicitis, Clinics., 69 (2014) 677-682.
35. H. Koksal, Total oxidant status, total antioxidant status, and paraoxonase activity in acute appendicitis, Turkish J. Trauma Emerg. Surg., 21 (2015) 139-42.
36. M. Işık, Y. Demir, M. Kırıcı, R. Demir, F. Şimşek, Ş. Beydemir, Changes in the anti-oxidant system in adult epilepsy patients receiving anti-epileptic drugs, Arch. Physiol. Biochem., 121 (2015) 97-102.
37. E.M.M. Ali, H.H. Shehata, R. Ali-Labib, L.M. Esmail Zahra, Oxidant and antioxidant of arylesterase and paraoxonase as biomarkers in patients with hepatitis C virus, Clin. Biochem., 42 (2009) 1394-1400.
38. U. Koltuksuz, E. Uz, S. Özen, M. Aydinç, A. Karaman, Ö. Akyol, Plasma superoxide dismutase activity and malondialdehyde level correlate with the extent of acute appendicitis, Pediatr. Surg. Int., 16 (2000) 559-561.
39. M. Abu-Hilal, M.J.W. McPhail, L. Marchand, C.D. Johnson, Malondialdehyde and superoxide dismutase as potential markers of severity in acute pancreatitis, J. Pancreas., 7 (2006) 185-192.
40. C. Sen Tanrikulu, Y. Tanrikulu, M. Zafer Sabuncuoglu, M. Akif Karamercan, N. Akkapulu, F. Coskun, Mean platelet volume and red cell distribution width as a diagnostic marker in acute appendicitis, Iran. Red Crescent Med. J., 16 (2014) 10211.