Mustafa YILDIZ, Hakan TERZİ, Süleyman CENKCİ, Behiye YILDIZ

Arpa Çeşitlerinin 2-D Protein Profilleri ve Antioksidant Savunma Sistemindeki Krom VI Teşvikli Değişimler

K rom birikimi, büyüme, d-aminolevülinik asit dehidrataz ALAD aktivitesi, fotosentetik pigment içeriği, prolin içeriği, malondialdehit MDA içeriği, antioksidant enzim aktivitesi ve 2-D protein profilleri üzerine Cr VI stresinin 0, 75, 150 ve 225 µM etkisi Cr-toleranslı Zeynelağa ve Cr-hassas Orza-96 arpa çeşitleri kullanılarak araştırılmıştır. Orza-96’ya göre Zeynelağa gövde dokuları önemli düzeyde daha yüksek Cr biriktirmiştir. Orza96’ya göre Zeynelağa gövde kuru ağırlığı, klorofil ve karotenoid içeriği daha düşük azalma göstermiştir. Cr VI , ALAD aktivitesini önemli düzeyde azaltmış olmasına rağmen, arpa çeşitlerinin ALAD aktiviteleri arasında tutarlı bir farklılık bulunmamıştır. Cr VI prolin ve MDA içeriğini arttırmış; fakat bu etki Orza-96’da daha belirgin olarak belirlenmiştir. Cr VI stresi süperoksit dismutaz SOD , guaiakol peroksidaz POD , askorbat peroksidaz APX ve katalaz CAT aktivitelerinde artışa neden olmuştur. Genel olarak, artan antioksidant enzim aktiviteleri açısından iki arpa çeşidi arasında belirgin farklılık belirlenmiştir. Bu araştırmanın sonuçları, Orza-96’ya göre Zeynelağa’nın Cr VI stresine karşı daha iyi savunma mekanizmalarına sahip olduğunu göstermektedir. Diğer taraftan, iki-yönlü jel elektroforezi ile analiz edilmiş protein profilleri 26 proteinin farklı şekilde eksprese edildiğini göstermiştir

Anahtar Kelimeler:

Arpa, Cr VI, Antioksidant savunma, 2-D protein profili

Chromium VI -Induced Alterations in 2-D Protein Profiles and Antioxidant Defence Systems of Barley Cultivars

The effects of Cr VI stress 0, 75, 150 and 225 μM on Cr accumulation, growth, d-aminolevulinic acid dehydratase ALAD activity, photosynthetic pigments content, proline content, malondialdehyde MDA content, antioxidant enzymes activity and 2-D protein profile were investigated using Cr-tolerant Zeynelağa and Cr-sensitive Orza-96 barley cultivars. Zeynelağa accumulated significantly higher Cr in shoots than Orza- 96. Zeynelağa had a lower reduction in shoot dry weight, chlorophyll and carotenoid contents than Orza-96. Although Cr VI significantly reduced the ALAD activity, there was no consistent difference between ALAD activities of barley cultivars. Cr VI increased proline and MDA contents, but this effect was more pronounced in Orza-96. Cr VI stress caused an increase in the activities of superoxide dismutase SOD , guaiacol peroxidase POD , ascorbate peroxidase APX and catalase CAT . There was, in general, a marked difference between two barley cultivars in the extent of increased antioxidant enzyme activity. The results of the present study indicated that Zeynelağa seems to have better defense mechanisms to Cr VI stress compared to Orza-96. On the other hand, protein profile analyzed by two-dimensional gel electrophoresis showed differential expression of 26 proteins.

Keywords:

Barley, Cr VI, Antioxidant defence, 2-D protein profile,

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