Kağan KILINÇ, Abdülkerim BEDİR, Nurol ARIK, Muhlise ALVUR, Ali Fuat BODUR

Esansiyel Hipertansiyonlu Hastalarda M235T Anjiotensinojen Gen Polimorfizmi

Esansiyel hipertansiyon, patogenezinde çeşitli genlerin rol oynadığı poligenik bir hastalıktır. Kan basıncının regülasyonunda görev yapan renin-angiotensin sistemi (RAS) aynı zamanda hipertansiyon gelişimine de neden olabilmektedir. Yapılan çalışmalar neticesinde angiotensinogen (AGT) genin genetik polimorfizm gösterdiği tespit edilmiştir. AGT’ nin en yaygın iki varyantı T174M ve M235T’ dir. Çeşitli populasyonlar üzerinde yapılan çalışmalar sonunda, M235T varyantının diğer varyantlara göre esansiyel hipertyansiyonla daha fazla ilişkili olduğu ifade edilmektedir. Bununla beraber, AGT gen polimorfizminin hipertansiyonla ilişkisi etnik farklılıklar göstermektedir. 105 hipertansif hasta ve 56 normotensif kişiyi kapsayan bu çalışmamızda, Türk populasyonunda AGT 235T varyantının allel frekansı ve esansiyel hipertansiyonla ilişkisi incelenmiştir. MS-PCR’ın (mutagenically seperated polymerase chain reaction) TD PCR (touchdown polimerase chain reaction) formatında kullanıldığında bu çalışmada 235T allel frekansı 0.43 olarak bulunuştur ki bu değer beyaz ırk için tespit edilen değerler ile uyumludur. Çeşitli çalışmalarda bu polimorfizmin esansiyel hipertansiyonla ilişkisi farklı sonuçlar göstermekte olup, çalışmamızda AGT 235T varyantının esansiyel hipertansiyonla anlamlı bir ilişkisi gözlemlenmemiştir (P=0.54). Bununla beraber çalışmamızda hipertansif grupta TT homozigot genotipli hastaların diğer genotipli hastalara (TM+MM) göre anlamlı derecede daha yüksek sistolik kan basıncına sahip oldukları gözlendi (p

Anahtar Kelimeler:

Esansiyel Hipetansiyon, Anjiotensinojen, M235T Polimorfizm, Touchdown PCR

Angiotensiınogen Gene Polymorphism in Patients with Essential Hypertension

Essential hypertension is a polygenic disease indicating that several genes are involved in its pathogenesis. The renin-angiotensin system, which is involved in the regulation of blood pressure, may play an important role in the development of hypertension. It is shown taht the angiotensinogen (AGT) gene had genetic polymorphism and and most common variants of AGT are T174M and M235T. In several studies on varies populations, it was observed that M235T variant is more related witch essential hypertension than other variants. However, the relation between AGT gene polymorphism and hypertension shows ethnic differences. We examined 105 hypertensive patients and 56 normotensive persons to estimate allel frequency of AGT 235T variant and to establish relationship with essential hypertension in Turkish population. In this study, orginally, mutagenically seperated (MS) PCR was used in the from of touch down (TD) PCR. Frequency of 235T allel was found 0.43, which accords to previous studies results in whites. Several studies were shown differences about their relation with essential hypertension. In our study we have not observed any significant relationship between the variant of AGT 235T and essential hypertension (P=0.54). However, we observed that hypertensive patients with homozygous T genotype have higher systolic blood pressure than hypertensive patients with other genotype (TM+MM, p<0.02).

Keywords:

Essential Hypertension, Angiotensinogen, M235T Polymorphism, Touchdown PCR,

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