Yanık hastalarından elde edilen Escherichia coli izolatlarının antibiyotik direnci

Yaygın olarak kullanılan antibiyotiklere karşı bakteriyel direnç, gelişmekte olan küresel bir sağlık sorunudur ve yanık hastalarında büyük bir probleme neden olmaktadır. Antimikrobiyal tedavilerdeki önemli gelişmelere rağmen, yanık hastalarında enfeksiyona bağlı ölüm oranı riski nispeten yüksektir. Escherichia coli, yanık yarası enfeksiyonlarının en yaygın etkenlerinden biridir. Bu nedenle bu çalışmada yanık yaralarından E. coli izolatlarının VITEK 2 sistemi kullanılarak tanımlanması, karakterize edilmesi ve en sık kullanılan antibiyotiklere karşı antibiyotik dirençlerinin disk difüzyon yöntemi ile test edilmesi amaçlanmıştır. Çalışmamızda yanık hastalarından alınan 147 klinik örneğin 25'inde (%17) E. coli bakterisi saptandı. Tüm bu izolatların sefalotin, sefradin, piperasilin ve rifampin antibiyotiklere dirençli olduğu bulundu. Amoksisilin+klavulanik asit ve ampisiline direnç %96 (± %8), bunu %92 (± %11) direnç oranıyla amikasin ve sefotaksim izledi. Öte yandan E. coli izolatlarına karşı en yüksek duyarlılığı gösteren antibiyotikler ise imipenem (%96 ± %8), tetrasiklin (%88 ± %13) ve gentamisin (%76 ± %17) olmuştur. Çoklu ilaca dirençli bakteriler, klinik uygulamalar için ana konulardan biridir, bu nedenle bunların karakterizasyonu, uygun bir tedavi stratejisi geliştirmede hayati önem taşır. Bu çalışma, E. coli'nin yanık yaralarında var olduğunu ve farklı antibiyotiklere direnci nedeniyle yara enfeksiyonuna neden olabileceği sonucuna varmıştır.

Anahtar Kelimeler:

Antibiyotik, Yanık yarası, Tanımlama, VITEK 2

Antibiotic resistance of Escherichia coli isolates obtained from burn patients

Bacterial resistance to widely used antibiotics is an emerging global health issue and causes a huge problem in burn patients. Despite important developments in antimicrobial treatments, the risk of infection-associated mortality rate in burn patients is comparatively high. Escherichia coli is one of the most common causative agents of burn wound infections. Therefore, this study aimed to identify and characterize E. coli isolates from burn wounds using the VITEK 2 system and to test their antibiotic resistance to the most commonly used antibiotics with the disc diffusion method. In our study, of 147 clinical samples obtained from burn patients, 25 (%17) were detected as positive for E. coli. All these isolates were found to be resistant to cephalothin, cephradine, piperacillin, and rifampin antibiotics. The resistance to amoxicillin+clavulanic acid and ampicillin was 96% (± 8%), which was followed by amikacin and cefotaxime with a 92% (± 11%) resistance rate. On the other hand, imipenem (96% ± 8%), tetracycline (88% ± 13%), and gentamicin (76% ± 17%) were the antibiotics that showed the highest sensitivity against E. coli isolates. The multidrug-resistant bacteria are one of the main issues for clinical applications, so their characterization is vital in developing a proper treatment strategy. This study concluded that E. coli exists in burn wounds and might cause wound infection due to its resistance to different antibiotics.

Keywords:

Antibiotic, Burn wound, Identification, VITEK 2,

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