Mikroalg yağının lipaz katalizli hidroliz tepkimesine etki eden proses parametrelerinin istatiksel yöntemle optimizasyonu

Birçok endüstriyel tesisin hammadde kaynağı olarak kullanılan yağların hidrolizi önemli bir kimyasal prosestir. Yağ asitleri yenilenebilir kaynaklardan üretilen yağların hidrolizi ile sentezlenir. Son yıllarda bitkisel ve hayvansal yağlara alternatif mikroalglerden elde edilen yağların hammadde kaynağı olarak kullanılması giderek yaygınlaşmaktadır. Bu çalışmada Design Expert bilgisayar programı yardımı ile Chlorella protothecoides mikroalg yağının Noopazyme lipazı katalizörlüğünde hidroliz tepkimesine etki eden parametrelerin (lipaz miktarı, sıcaklık, yağ/su (g/g)) etkileri incelenerek yüksek dönüşümle serbest yağ asitleri (SYA) elde etmek amacı ile optimizasyonu yapılmıştır. Deneysel çalışmalar sonucunda en yüksek SYA içeriği (%98), lipaz miktarı %15, yağ/su oranı (g/g) 0.20 ve sıcaklık 55°C olduğu koşullarda elde edilmiştir. Ayrıca Noopazyme lipazının 4 kez tekrar kullanılması sonucunda aktivitesinin %40’ını kaybettiği belirlenmiştir.

Anahtar Kelimeler:

Alg yağı, Chlorella protothecoides, Hidroliz, Lipaz, Yanıt yüzey yöntemi

Statistical optimization of hydrolysis reaction process parameters by lipase catalysis of microalgae oil

Hydrolysis of oils used as the raw material source of many industrial plants is an important chemical process. Fatty acids are synthesized by hydrolysis of oils produced from renewable sources. In recent years, the use of oils derived from microalgae as an alternative to vegetable and animal oils has become commonly increasing. In this study, the effects of the parameters (lipase amount, temperature, oil / water (w/w)) of Chlorella protothecoides microalgae oil were analyzed to obtain high conversion of free fatty acids with the Design Expert computer program. As a result of the experimental studies, the highest FFA content (98%) was obtained under the conditions where lipase amount was 15%, oil/water ratio (g/g) 0.20 and temperature was 55°C. In addition, as a result of 4 reuse of Noopazyme lipase, it was determined that it lost 40% of its activity.

Keywords:

Algae oil, Chlorella protothecoides, Hydrolysis, Lipase, Response surface methodology,

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