Gökkuşağı alabalığı gonad hücre hattı-2 (RTG-2) üzerinde Eruca vesicaria'dan elde edilen gümüş nanoparçacıkların sitotoksisitesi

Gümüş (Ag) nanoparçacıklarının gıda, ilaç, tarım gibi birçok sektörde artan kullanımı ekolojik çevre üzerinde toksik etkilere yol açmaktadır. Bu nedenle Ag nanoparçacık sentez yöntemlerinin neden olduğu toksisiteyi azaltmak için biyolojik sentez yöntemleri üzerinde çalışmalar yapılmaktadır. Bununla birlikte, balık hücre hatlarında biyosentezlenmiş Ag nanoparçacıklarının toksisitesi üzerine yapılan çalışmalar çok azdır. Bu çalışmanın amacı, Eruca vesicaria bitki özü ile Ag nanoparçacıklarının biyolojik sentezini gerçekleştirmek ve gökkuşağı alabalığı gonad hücre hattı-2'de (RTG 2) toksisitelerini incelemektir. E. vesicaria'dan elde edilen Ag nanoparçacıklarının karakterizasyonu UV-vis, TEM ve XRD ile yapılmıştır. Ag nanoparçacıklarının (100 µg/mL- 6,25 µg/mL) 24 saat boyunca RTG-2 hücresindeki toksisitesi sülforodamin B tahlili ile belirlendi. Küre, üçgen, küp ve 5-20 nm boyutlarında elde edilen Ag nanopartiküller, p≤0,001 seviyelerinde doza bağlı olarak RTG-2 balık hücreleri üzerinde önemli toksik etki göstermiştir. Bu çalışma, biyolojik sentez yoluyla elde edilen Ag nanoparçacıklarının balık hücre hatları üzerinde toksik etkisinin olduğunun kanıtlanması ve sentez yöntemlerinden ziyade Ag nanoparçacıklarının çevreye salınımını azaltacak çalışmalara ihtiyaç olduğunu göstermesi açısından önemlidir.

Anahtar Kelimeler:

Biyolojik sentez, Sitotoksisite, Eruca vesicaria, RTG-2 hücre hattı, Gümüş nanoparçacık

Cytotoxicity of silver nanoparticles obtained from Eruca vesicaria on rainbow trout gonad cell line-2 (RTG-2)

The rising application of silver (Ag) nanoparticles in many sectors such as food, medicine, and agriculture lead to toxic effects on the ecological environment. Thus, studies on biological synthesis methods are carried out in order to diminish the toxicity caused by Ag nanoparticle synthesis methods. However, studies on the toxicity of biosynthesized Ag nanoparticles on fish cell lines are very few. The purpose of this research was to perform the biological synthesis of Ag nanoparticles via Eruca vesicaria plant extract and to examine their toxicity in rainbow trout gonad cell line-2 (RTG 2). The characterization of Ag nanoparticles obtained from E. vesicaria was done by UV-vis, TEM, and XRD. The toxicity of Ag nanoparticles (100 µg/mL- 6,25 µg/mL) in the RTG-2 cell for 24 hours was determined by sulforhodamine B assay. Ag nanoparticles obtained in the form of a sphere, triangle, cube, and sizes of 5-20 nm showed significant a toxic effect on RTG-2 fish cells depending on the dose at p≤0,001 levels. This study is important in terms of proving that Ag nanoparticles obtained by biological synthesis have a toxic effect on fish cell lines and showing that there is a need for studies to reduce the release of Ag nanoparticles to the environment rather than synthesis methods.

Keywords:

Biological synthesis, Cytotoxicity, Eruca vesicaria, RTG-2 cell line, Silver nanoparticle,

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