Mürit AKAL, Muhammed Emre DEMİRDERE, Derya ÜNLÜ

Anti-kanser ilacı 5-Fluorourasil'in bentonit yüklü kitosan/hidroksipropil metilselüloz biyonanokompozit filmlerden kontrollü salımı

Kontrollü salım, geleneksel tedavinin aksine, bir maddenin bir ortamdaki derişimini belirli bir süre boyunca korumak için kademeli olarak bir sistemden serbest bırakılması anlamına gelmektedir. İlaç salım sistemlerinde ilacın hastalıklı bölgeye kontrollü bir şekilde salımı, tedavi ve toksisite açısından önem arz etmektedir. Özellikle 5-Fluorourasil (5-FU) gibi toksik özelliği fazla olan ilaçlar kontrollü ilaç salım teknolojisinde özel yere sahiptir. Tedavinin başarılı bir şekilde yapılabilmesi için 5-FU gibi antikanser ilaçlarının olası yan etkilerini ortadan kaldırmak ve tedavi edici etkisini arttırmak için kontrollü ilaç salım sistemlerinin geliştirilmesi önem kazanmaktadır. Bu çalışmanın amacı, 5-FU'in kontrollü salımı için biyolojik olarak parçalanabilen ve biyouyumlu özgün bir biyonanokompozit film geliştirmektir. Bu amaçla bentonit yüklü kitosan hidroksipropil metilselüloz biyonanokompozit filmler çözeltiden döküm ve solvent buharlaştırma tekniği kullanılarak sentezlenmiştir. Biyonanokompozit filmlerin kimyasal bağ yapısı, bentonit ilavesinin yapıdaki amin ve hidroksil gruplarına etkisi Fourier dönüşümlü kızılötesi ile belirlenmiştir. Polimerlerin ve bentonitin morfolojik özellikleri ve uyumluluğu, taramalı elektron mikroskobu kullanılarak karakterize edilmiştir. Biyonanokompozit filmlerin sorpsiyon derecesi şişme testleri ile değerlendirilmiştir. Nanokompozit filmdeki bentonit içeriğinin %5'ten %25'e artması ile sorpsiyon derecesi %2.99'dan % 65.05'e artmıştır. Ayrıca sentezlenen biyonanokompozit filmlere bentonit ilavesinin ve pH'ın 5-FU'in yüklenmesi ve enkapsülasyon verimliliği üzerindeki etkileri araştırılmıştır. 5-FU yüklenen nanokompozit filmlerden ilacın kontrollü salım çalışmaları yapılmıştır. Elde edilen sonuçlar, nanokompozit ilaç taşıyıcı filmlerde bentonit kullanımının kapsülleme verimliliğini ve salım özelliklerini geliştirdiğini göstermiştir. %5 bentonit yüklü filmde dört günün sonunda pH=7.4'te %40.12 salım değeri elde edilmiştir.

Anahtar Kelimeler:

Bentonit, Fluorourasil, Hidroksipropil metilselüloz, İlaç salımı, Kitosan, Nanokompozit film

Controlled release of the anti-cancer drug 5-Fluorouracil from bentonite-loaded chitosan/hydroxypropyl methylcellulose bionanocomposite films

Controlled release, on the contrary traditional treatment, means the gradual release of a substance from a system in order to maintain its concentration in a medium over a period of time. In drug release systems, the controlled release of the drug to the diseased area has importance in terms of treatment and toxicity. Especially drugs with high toxic properties such as 5-Fluorouracil (5-FU) have an important place in controlled drug release technology. In order to complete the treatment successfully, it is important to develop controlled drug release systems to eliminate the possible side effects of anticancer drugs such as 5-FU and to increase the therapeutic effect. The aim of this study is to develop a unique biodegradable and biocompatible bionanocomposite film for the controlled release of 5-FU. For this purpose, bentonite loaded chitosan hydroxypropyl methylcellulose bionanocomposite films were synthesized using solution casting and solvent evaporation techniques. The chemical bond structure of the bionanocomposite films and the effect of bentonite addition on the amine and hydroxyl groups in the structure were determined by Fourier transform infrared. Morphological properties and compatibility of polymers and bentonite were characterized by using scanning electron microscopy. The degree of sorption of the bionanocomposite films was evaluated by swelling tests. As the bentonite content in the nanocomposite film increased from 5% to 25%, the sorption degree of film increased from 2.99% to 65.05%. In addition, the effects of bentonite addition of the synthesized bionanocomposite films and pH of medium on the 5-FU loading and encapsulation efficiency were investigated. Controlled release studies were carried out by using 5-FU loaded nanocomposite films. The obtained results showed that the usage of bentonite in nanocomposite drug carrier films were improved the release properties. After four days, a release value was obtained as 40.12% at pH=7.4 in the 5 wt. % bentonite loaded films.

Keywords:

Bentonite, Fluorouracil, Hydroxypropyl methylcellulose, Drug release, Chitosan, Nanocomposite film,

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