Etem OSMA, Nuray GÜLOĞLU, Eda TÜRKOĞLU, Müjgen ELVEREN

Acetaminophen (Paracetamol) ve Gemfibrozil’in Farklı Buğday Varyetelerinin Mineral Alımı ve H2O2 İçeriği Üzerine Etkileri

Bu çalışma ile Tıbbi İlaç ve Kişisel Bakım Ürünleri (PPCPs)’nin Ahmetağa, Cemre ve Michelangelo buğday varyeteleri üzerindeki etkisi araştırılmıştır. Araştırmada günlük hayatta sıklıkla tüketilen acetaminophen ve gemfibrozil olmak üzere iki farklı tıbbi ilaç etken maddesi kullanılmıştır. Çalışma için ekim yapılacak toprağa, 50, 100 ve 250 mg olacak şekilde acetaminophen ve gemfibrozil karıştırılmıştır. İlaç etken maddesi ile karıştırılmış 650 g toprağın üzerine her bir varyeteden 7 g buğday tohumu ekilmiş, bunun üzeri ise 100 g toprak ile kaplanmıştır. Tarla kapasitesine uygun olarak buğdaylar belirli aralıklarla sulanarak, 15 gün yetiştirildikten sonra hasat edilmiştir. Hasat edilen örneklere ekstraksiyon işlemi yapıldıktan sonra, örneklerde H2O2 içeriği belirlenmiştir. Ayrıca, buğday örnekleri ön işlemlerden geçirildikten sonra mineral element konsantrasyonları ICP-OES’te analiz edilmiştir. Kontrol örnekleri ile test örnekleri arasındaki ilişki %95 güven aralığında, istatistiksel olarak ANOVA testi ile değerlendirilmiş, anlamlı farklılıklar tespit edilmiştir. Çalışmada, acetaminophen ve gemfibrozil uygulanan örneklerde konsantrasyon artışına bağlı olarak kontrol grubu ile kıyaslandığında buğdayların mineral element alımında azalma gözlenirken, H2O2 içeriğinde artış olduğu belirlenmiştir.

Anahtar Kelimeler:

Acetaminophen, Buğday, Gemfibrozil, ICP-OES, Mineral element

Effects of Acetaminophen (Paracetamol) and Gemfibrozil on Mineral Uptake and H2O2 Content of Different Wheat Varieties

This study investigated the effects of Pharmaceuticals and Personal Care Products (PPCPs) on wheat varieties of Ahmetağa, Cemre and Michelangelo. Two different active pharmaceuticals substances acetaminophen and gemfibrozil, which are frequently consumed in daily life, were used in the study. For study, acetaminophen and gemfibrozil were mixed with soil as follows: 50, 100, and 250 mg. 7 g of wheat seeds were planted from each varieties on each 650 gr of soil that mixed with the pharmaceuticals substance, then covered with 100 gr of soil. The wheat was irrigated at certain intervals in accordance with the field capacity and harvested after 15 days. After extracting the harvested wheats, the content of H2O2 in the samples were determined. Moreover, wheat samples were analyzed by ICP-OES for mineral element concentrations after pre-treatment. The relationship between control samples and test samples was evaluated statistically at 95% confidence interval, and significant differences were determined. In the study, it was determined that there was an increase in the H2O2 content in parallel with a decrease in mineral element uptake of wheat samples treated with increase amount of acetaminophen and gemfibrozil when compared to the control group.

Keywords:

Acetaminophen, Gemfibrozil, ICP-OES, Mineral element, Wheat,

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