STANDART VE HİPOALERJENİK BEBEK SÜTÜ FORMÜLLERİNİN ATR-FTIR SPEKTROSKOPİSİ VE ÇOK DEĞIŞKENLİ ANALİZ YÖNTEMLERİYLE İNCELENMESİ

Bebek formülleri, bebeklerin beslenme gereksinimlerini karşılamak için tasarlanmış besinler ve biyoaktif bileşenler içerir. Bebek formüllerinin çoğu inek sütü (CM) bazlı normal formüllerdir. CM en önemli alerjen kaynaklarından biri olduğu için CM alerjisi olan bebekler için kısmen ve yoğun hidrolize, aminoasit bazlı gibi özel formüller bulunmaktadır. Bu çalışmada, Türkiye’de ticari olarak satılan standart ve hipoalerjenik bebek sütü formüllerinin moleküler farklılıklarının ayırt edilebilmesi amacıyla zayıflatılmış toplam yansıma (ATR)- Fourier Dönüşümlü Kızılötesi (FTIR) spektroskopisi, kemometrik yöntemlerle birlikte kullanılmıştır. Hipoalerjenik formül (HF) örneklerinde standart formüllere (RF) göre önemli derecede daha yüksek doymuş yağ asidi miktarı ve niteliksel olarak daha uzun zincirli yağ asidi gruplarının olduğu bulunmuştur. Hiyerarşik kümele analizi (HCA) ve temel bileşen analizi (PCA) sonuçlarına göre, %100 duyarlılık ve %83 varyasyon ile en belirgin ayrımın olduğu spektral bölge 1200-800 cm-1 olarak belirlenmiştir.

Anahtar Kelimeler:

Bebek sütü formülü, hipoalerjenik formül, ATR-FTIR spektroskopisi, çok değişkenli analiz yöntemleri

INVESTIGATION OF REGULAR AND HYPOALLERGENIC INFANT MILK FORMULAS BY ATR-FTIR SPECTROSCOPY COMBINED WITH MULTIVARIATE ANALYSIS METHODS

Infant formulas contain nutrients and bioactive ingredients designed to meet the nutrition requirements of infants. The majority of infant formulas are cow's milk (CM) based regular ones. Since CM is one of the most important allergen source, there are specialized formulas such as partially and extensively hydrolyzed, aminoacid‐based for CM‐allergic infants. In the current study, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) in combination with multivariate analysis was used to identify the molecular differences between commercially available hypoallergenic (HF) and regular formulas (RF) in Turkey. Higher saturated fatty acid (FA) content and qualitatively longer acyl chained FAs were observed in hypoallergenic formulations. The results of hierarchical cluster analysis (HCA) and principal component analysis (PCA) showed that there were differences between the infrared spectra of the two groups. The HF and RF samples were clearly separated from each other in 1200-800 cm-1 spectral region with 83% variation and 100% accuracy.

Keywords:

Infant milk formula, hypoallergenic formula, ATR-FTIR spectroscopy, multivariate analysis methods,

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