Saeid CHOBDAR RAHIM, Janan HOSSEİN ZADEH, Fikret PAZIR, Gülden OVA

FARKLI ÇÖZGEN VE ÇÖZGEN KARIŞIMLARI İLE EKSTRAKSİYONUN SAFRANIN (Crocus sativus L.) TOPLAM BİYOAKTİF BİLEŞEN MİKTARINA ETKİSİ

Safran Standardına göre (TS/ISO 3632, 2011) kalite açısından birinci derece bir safran baharatının, 13 farklı çözgen sistemi (saf su, metanol, etanol, asetonun farklı oranlarda karışımı) kullanılarak elde edilen ekstraktlarının toplam fenolik ve flavonoid madde miktarlarıyla antioksidan aktiviteleri üzerine etkileri incelenmiştir. Sonuçlar incelendiğinde en yüksek toplam fenolik madde miktarı 2265.00 mg GAE/100 g KM ile su/etanol/metanol (v/v/v, 30/30/40) ekstraktında saptanırken, en düşük toplam fenolik madde miktarı 40.00 mg GAE/100 g KM ile aseton ekstraktında saptanmıştır. Toplam flavonoid madde açısından en yüksek miktar 587.14 mg KE/100 g KM ile su/etanol/metanol (v/v/v, 30/30/40) ekstraktında tespit edilirken, aseton ekstraktında flavonoid bileşeni tespit edilememiştir. Antioksidan aktivite açısından DPPH ve ABTS+ analiz yöntemlerinde en yüksek değerler sırasıyla 1202.68 mg TE/100 g KM ve 1383.74 mg TE/100 g KM ile su/etanol/metanol (v/v/v, 30/30/40) ekstraktında saptanırken, en düşük miktar DPPH için 232.43 mg TE/100 g KM etanolde ve ABTS+ için 251.23 mg TE/100 g KM aseton ekstraktında saptanmıştır.

Anahtar Kelimeler:

Safran, Ekstraksiyon, Toplam Fenolik Madde, Flavonoid Madde, Antioksidan Aktivite, Antioxidant Activity

THE EFFECT of EXTRACTİON WİTH DİFFERENT SOLVENTS and SOLVENT MİXTURES on THE TOTAL AMOUNT of BIOACTIVE COMPONENTS of SAFFRON (Crocus sativus L.)

This study examines various activities of the saffron, first quality according to Saffron Standard (TS/ISO 3632, 2011) extracts by using 13 different solvent systems, (distilled water, methanol, ethanol, acetone and their mixtures at different levels). Total phenolic measured in water, ethanol, methanol, (v/v/v, 30/30/40) extract with 2265.00 mg GAE/100 g DM as highest and, the acetone extract with 40.00 mg GAE/100 g DM as lowest. The highest amount of total flavonoids was detected in water, ethanol, methanol (v/v/v, 30/30/40) extract with 587.14 mg CE/100 g DM, flavonoid component was not detected in acetone extract. As antioxidant activity in DPPH and ABTS+ analysis methods, the highest values were detected in water, ethanol, methanol (v/v/v, 30/30/40) extract with 1202.68 mg TE/100 g DM and 1383.74 mg TE/100 g DM, respectively. The lowest were detected 232.43 mg TE/100 g DM (DPPH) in ethanol and 251.23 mg TE/100 g DM (ABTS+) in acetone extract.

Keywords:

Saffron, Extraction, Total Phenolic Content, Flavonoid Content,

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