Hatice Reyhan OZİYCİ, İrfan TURHAN, Nedim TETİK2, Asli Arslan KULCAN, Tugba AKKOYUN, Mustafa GERMEC, Mustafa KARHAN, Ercan YATMAZ

CONCENTRATION OF D-PINITOL in CAROB EXTRACT by USING MULTI-STAGE ENRICHMENT PROCESSES

D-pinitol insülin mekanizması ile ilişkili hastalıkların tedavisinde kullanılabilme potansiyeli olan bir şeker alkolüdür. Bu şeker alkolü bakımından zengin olan keçiboynuzu meyvesi, D-pinitolün ayrıştırılması ve saşaştırılması amacıyla son yıllarda gerçekleştirilen birçok araştırmada materyal olarak kullanılmıştır. Bu çalışmada keçiboynuzu ekstraktının D-pinitol içeriğini konsantre etmek amacıyla farklı zenginleştirme prosesleri kullanılmıştır. Hedef bileşiğin konsantre hale getirilmesi amacıyla kullanılan tekniklerin etkinliğini belirlemede keçiboynuzu ekstraktında bulunan diğer safsızlıkların (esas olarak şekerlerin) uzaklaştırılması önemli bir gösterge olarak kullanılmıştır. Elde edilen bulgulara göre; D-pinitol konsantrasyonundaki maksimum artış etanol fermantasyonu, membran filtrasyonu ve solvent ekstraktsiyonu gibi tekniklerin beraber uygulanmasıyla elde edilmiştir. Bu çok aşamalı prosesin sonunda, keçiboynuzu ekstraktının sahip olduğu başlangıç D-pinitol miktarı (9.38 g/100 mL kuru madde) yaklaşık dört kat konsantre (37.53 g/100 mL kuru madde) hale getirilmiştir.

KEÇİBOYNUZU EKSTRAKTINDA BULUNAN D-PİNİTOLÜN ÇOK AŞAMALI ZENGİNLEŞTİRME PROSESİ İLE KONSANTRASYONU

Abstract D-pinitol, a cyclic sugar alcohol, is claimed to be a potential therapeutic compound related to the illnesses arising from insulin mechanism. Carob is a rich source of this compound and has recently begun to be used in different separation and purification studies for obtaining D-pinitol. In this study, different enrichment processes were applied to concentrate the D-pinitol content of the carob extract. To determine the effectiveness of the processes applied for concentration of the target compound in carob extract, removal of other impurities (mainly sugars) was used as an important indicator. According to the results; the highest increase in D-pinitol concentration was observed in the enrichment process combining the techniques such as ethanol fermentation, membrane filtration and solvent extraction. At the end of this multi-stage process, D-pinitol concentration increased approximately four-fold (37.53 g/100 mL dry weight) when compared to its initial level (9.38 g/100 mL dry weight) in carob extract.

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