Karanlıkla Teşvik Edilen Yaprak Senesensi Sürecinde Gibberellik Asit ve 6- Benzilaminopürinin Bazı Biyokimyasal Parametreler Üzerine Etkisi

Bu çalışmada uzun süre karanlık ortamda bekletilerek senesens süreci uyarılan Tropaeolum majus L. (Latin çiçeği) yapraklarına, gibberellik asit (GA3) ve 6-benzilaminopürin (BAP) uygulamanın bazı senesens parametrelerine etkisi incelenmiştir. Bu amaçla 10-5 M BAP ve 10-5 M GA3 uygulanarak karanlık ortama bırakılan yaprakların klorofil, protein, nişasta ve hidrojen peroksit miktarları ile katalaz ve peroksidaz enzimlerinin aktiviteleri belirlenmiştir. Bu iki hormon yaprakların klorofil ve protein içeriğinde meydana gelen kaybı azaltırken nişasta içeriğindeki düşüşü engelleyememiştir. BAP ve GA katalaz aktivitesini kontrole göre anlamlı şekilde artırırken peroksidaz aktivitesini azaltmıştır. Hidrojen peroksit içeriği BAP ve GA3 uygulanan yapraklarda belirgin şekilde azalmıştır. Bu sonuçlar, BAP ve GA3 uygulamanın yaprak senesensinin biyokimyasal parametrelerini değiştirerek bu sürecin geciktirilmesinde etkili olduğunu göstermektedir.

Effects of Gibberellic Acid and 6-Benzylaminopurine on Some Biochemical Parameters During Dark-Induced Leaf Senescence

In this study, effects of gibberellic acid (GA3) and 6-benzylaminopurine (BAP) on some senescence parameters in detached leaves of Tropaeolum majus L. (Nasturtium) during dark-induced senescence were investigated. For this purpose, chlorophyll, protein, starch, hydrogen peroxide amounts, catalase and peroxidase activities of leaves kept in darkness after applying 10-5 MGA3 and 10-5 M BAP were determined. Both the hormones reduced losses in chlorophyll and protein contents of leaves, but these treatments did not prevent rapid losses in starch amount under dark conditions. Applications of BAP and GA3 increased significantly the catalase activity, decreased meaningfully peroxidase activity compared to the control group. The contents of hydrogen peroxide were markedly decreased in leaves applied by BAP and GA3. These results suggest that applications of BAP and GA3 found to be effective in senescence delay by changing the biochemical parameters of leaf senscence.

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