Suna KALENDER, Ayten ÇELEBİ, Turan GÜVEN

Sodyum metabisülfit' in fare dermal mast hücrelerindeki degranülasyon etkisi

Bu çalışmada, sodyum metabisülfitin 0.30 mg/kg ve 1.0 mg/kg'lık dozları farelerin (Muş musculus domesticus) deri altına enjekte edildikten 1, 3, 6 ve 12 saat sonra dermal mast hücreleri ve bu hücrelerde degranülasyon etkisi transmisyon elektron mikroskobu (TEM) ile incelenmiştir. Sodyum metabisülfitin 0.30 mg/kg'lık dozunun deri altına enjeksiyonundan 1, 3 ve 6 saat sonra dermal mast hücrelerinde herhangi bir değişiklik meydana gelmemiştir. Fakat enjeksiyondan 12 saat sonra dermal mast hücrelerinin bazı granüllerinde çözülme, bazılarında da kısmi bir boşalma gözlenmiştir. Sodyum metabisülfitin 1 mg/kg'lık dozunun deri altına enjeksiyonundan 1 saat sonra dermal mast hücrelerinde herhangi bir degranülasyon gözlenmemiştir. Bununla beraber enjeksiyondan 3 saat sonra yaygın olmayan degranülasyon, 6 saat sonra dermal mast hücrelerinin genel görünümü ve sitoplazmik zarının bütünlüğünde bozulma görülmüştür. Buna ilaveten 12 saat sonra da herhangi bir degranülasyon tespit edilmemiştir.

Degranülation effect of sodium metabisulfite on the dermal mast cells of mouse

In this study, the degranulation effect of intradermally injected various sodium metabisulfite doses (0.30 mg/kg and 1.0 mg/kg) on the dermal mast cells of mouse {Mus musculus domesticus) have been examined after injection 1, 3, 6 and 12 hours by transmission electron microscope (TEM). No change on the dermal mast cells was observed 1, 3 and 6 h after injection of sodium metabisulfite (0.30 mg/kg). However, after 12 h from injection, dissolution at some granules and a partial degranulation of dermal mast cells were observed. No degranulation on the dermal mast cells was observed 1 h after injection of sodium metabisulfite (1mg/kg). Howtver, there were some degranulation after 3 hours. The general appearance of dermal mast cells and whole of cytoplasmic membrane were damaged after 6 h from injection, hi addition to that, after 12 h from injection, no degranulation was observed.

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