Mahmut SELVİ, Rabia SARIKAYA, Figen ERKOÇ

Acute behavioral changes in the guppy (Proecilia reticulata) exposed to temephos

Temefos sivrisinek, tatarcık ve karasinek larvalanyla mücadelede kullanılan organofosforlu insektisitlerden biridir. Bu çalışma temefosun akut toksisitesinin lepistes (Poecilia reticulata) bireylerindeki davranış değişimlerinin belirlenmesini amaçlamaktadır. Denemelerde kullanılmak üzere lepistes balıklan (Poecilia reticulata) seçilmiştir. Her bir konsantrasyondaki davranış değişikleri kaydedilmiştir. Deneyler üç kez tekrarlanmıştır. Temefosun yetişkin erkek lepisteslerde 96 saatlik akut toksisite değeri 10 ila 100 mg/1 (ortalama >50 mg/1) arasındadır. 100 mg/1 dozda, 96 saat sonunda tüm balıklar ölmüştür. Buna ilave olarak, en düşük konsantrasyon olan 1 mg/1' de davranış değişikliği bulunmamıştır. Denemeler süresince su sıcaklığı 21±l°C'de sabit tutulmuştur. Ayrıca insektisitin herbir konsantrasyonunda balıklarda meydana gelen davranış değişimleri de gözlenmiştir.

Temefosa maruz kalan lepistes (Poecilia reticulata) bireylerindeki akut davranış değişimleri

Temephos is an organophosphorus insecticide used to control mosquito, midge and black fly larvae. This study was aimed to determine the acute toxicity of temephos on behavior of the guppy (Poecilia reticulata). Guppy fish (Poecilia reticulata) were selected for the bioassay experiments. Behavioral changes at each temephos concentration were recorded. The experiments were repeated 3 times. The 96 h acute toxicity range of temephos to adult male guppies was within 10 and 100 mg/1 (mean >50 mg/1). At 100 mg/1 dose, all fish died within 96 hours. In addition, no changes in behavior were observed at the lowest concentration of 1 mg/1. Water temperature was regulated at 21±l°c. Moreover, behavioral changes at each temephos concentration were observed for the individual fish.

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1. "Hazardous Substance Data Base"., TOXNET. National Library of Medicine, Bathesda, MD (1993).
2. Pierce, R.H., R.B. Brown, K.R. Hardman, M.S. Henry, C.L. Palmer, T.W. Miller and G. Witcherman., "Fate and Toxicity of Temephos Applied to an Intertidal mangrove Community'Voarna/ of the American Mosquito Control Association. December, 5 (4): 569-578 (1989).
3. Ardalı, Y., "Endüstriyel Atık Sulardan Ağır Metallerin Adsorbsiyon ile Uzaklaştırılması" "Heavy Metal Expurgation from Industrial Waters by Absorption", MS. Thesis, Ondokuz Mayıs Üniversitesi Fen Bilimleri Ens., Samsun (In Turkish), (1990).
4. Brando, C, Bohets, H.L., Vvyer, I.E. and Dierickx, P.J., "Correlation Between the In vivo Cytotoxicity to Cultured Fathead Minnow Fish Cells and Fish Lethality Data for 50 Chemicals", Chemosphere, 25 (2): 553-62 (1992).
5. OECD (Organization for Economic Co-operation and Development), "OECD Guidelines for Testing of Chemicals",OECD, Paris, (1993).
6. Castano, A., Cantarino, M.J., Castillo, P. and Tarazona, J.V., "Correlation Between the RTG-2 Cytotoxicity Test EC50 and In vivo LC50 Rainbow trout Bioassay", Chemosphere. 32 (11): 2141-57 (1996).
7. APHA, AWWA, WEF, "Standard Methods for the Examination of Water and Wastewater", Washington, D.C.(1998).
8. Turkish Official Gazette (Resmi Gazete), "Su Kirliliği ve Kontrolü Yönetmeliği Numune Alma ve Analiz Metodlan Tebliği. Zehirlilik Seyreltme Faktörü (ZSF) Tayini",Tarih: 7.1.1991, Sayı: 20106 (1991).
9. Anadu, D.I., Anaso, H.U. and Onyeka, O.N.D., "Acute toxicity of the insect larvacide Temephos on the fish Tilapia melanopleura and Dragonfly larvae Neurocordella virginensis.", /. Environ. Set Health, b31 (6): 1363-1375 (1996).
10.Mayer, F.L. and Ellersieck, M.R., Manual of Acute Toxicity: Interpretation and Data Base for 410 Chemicals and 66 Species of Freshwater Animals, United States Department of the Interior Resource Publication (U.S. Fish and Wildlife Service), Washington D.C, 442-444 (1986).
11.Richmonds, C. and Dutta, H.M., "Effect of Malathion on the Optomotor Behavior of Bluegill Sunfish, Lepomis macrochirus", Comp. Biochem. Physiol., 102 (3), 523-26 (1992).
12.Sancho, E., Ferrando, M.D., Gamon, M., Andreu-Moliner, E., "Uptake and Elemination Kinetics of a Pesticide in the Liver of the European Eel" J Environ Sçi Health Part B 33:83-98 (1998).
13.Ferrando, M.D., Sancho, E. and Moliner, E.A., "Comparative Acute Toxicities of Selected Pesticides to Anguilla anguilla", Environ. Sci. Heath., B26 (5-6): 491-8 (1991).
14."World Health Organization", Temephos, Environmental Health Criteria 29, World Health Organization, Genava, (1984).