Öznur MERTOĞLU, Gürsel BİBEROĞLU, Ayşe BİLGİHAN, Muharrem POLAT, Yıldız GÜNEY

Total malondialdehyde levels and superoxide dismutase activities in the sera of guinea pigs after whole-body irradiation

Amaç: Bu çalışmada, kobayların 8 Gray (Gy) ve 15 Gy dozlarda, tüm vücut ışınlanmalarından 24 saat sonra, ışınlamanın seruma yansıyan akut etkilerini gözlemlemek için total malondialdehit (MDA) düzeyleri ve süperoksit dismutaz (SOD) aktivitelerinin ölçülmesi amaçlanmıştır. Gereç ve Yöntem: Kobaylar 8 Gy (n=10) ve 15 Oy (n=10) dozlarda, tek doz, tüm vücut ışınlamasına maruz bırakılmışlardır. Işınlamadan 24 saat sonra kan örnekleri alınmış ve bu örneklerden elde edilen serumlarda total MDA düzeyleri yüksek basınçlı sıvı kromatografısi (HPLC) yöntemiyle ve SOD aktiviteleri ise spektrofotometrik olarak ölçülmüştür. Aynı ölçümler ışınlama uygulanmayan kobaylarda da (n=10) yapılmıştır. Sonuçlar: 8 Gy dozla ışınlanan kobay grubunda, serum total MDA düzeyleri kontrole göre anlamlı (p 0,05) izlenmemiştir. Yorum: Bu çalışma sonucunda, serum MDA düzeylerinin doza bağlı olarak değişmesi, ışınlamanın akut etkisini izlemek açısından serum MDA düzeylerinin seçilebileceğini göstermektedir.

İyonize radyasyon uygulanan kobaylarda serum total malondialdehit düzeyleri ve süperoksit dismutaz aktiviteleri

Purpose: The aim of this study was to investigate the acute effects of whole-body irradiation in doses of 8 Gray (Gy) and 15 Gy, to malondialdehyde (MDA) levels and superoxide dismutase (SOD) activities in the sera of guinea pigs, 24 hours after irradiation. Methods: Guinea pigs were irradiated at doses of 8 Gy (n=10) and 15 Gy (n=10). Twenty-four hours after a single whole-body irradiation, serum total MDA levels were measured by high pressure liquid chromatography (HPLC) and SOD activities were measured using spectrophotometric techniques. These parameters were also evaluated in nonirradiated guinea pigs (n=10). Results: Serum total MDA levels were significantly lower (p0.05) with respect to the control group. In both of the irradiated groups, SOD activities did not change (p>0.05) in comparison with the control group. Conclusion: The results suggest that serum MDA levels are a useful parameter for monitoring the acute effects of irradiation since they vary depending on irradiation dose.

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