Rabet GÖZİL, Meltem BAHÇELİOĞLU, Engin ÇALGÜNER, Deniz ERDOĞAN, Çiğdem ELMAS

Sihler staining technique in the study of tongue innervation in the rat

Amaç: Sinirleri ve dallanmalarını ayrıntılı bir şekilde gösteren Sihler boyama tekniğiyle farklı kraniyal sinirlerle innerve edilen dilin innervasyonu gösterilmiştir. Metod: Sıçan dilininduyu ve motor sinir innervasyonunu göstermek amacıyla modifiye Sihler boyama tekniği kullanılmıştır. Bulgular: Dil kaslarında n.hypoglossus'un dağılımı gösterildi. Dilin 1/3 arka kısmına n.glossopharyngeus'un duyu liflerinin dağılımı izlendi. Ayrıca, n.lingualis ve dallarının corpus linguae'dan apex linguae'ya doğru olan seyri ve dallanması gösterildi. Sonuçlar: Uzun bir süredir gözardı edilmiş olan bu boyama tekniğinin yapılardaki karmaşık sinir dallanmalarını ve reinnervasyon deneylerinin sonuçlarını göstermesi bakımından önemli bir yöntem olacağı kanısındayız.

Sihler boyama tekniği ile rat dil innervasyonunun incelenmesi

Purpose: The tongue is a complex muscular organ constituted of several intrinsic and extrinsic muscles, innervated by several cranial nerves. Methods: In this study, the nerves and their branches in the tongue were demonstrated by Sihler's stain technique. Results: The distributions of the hypoglossal nerve in the muscles of the tongue were visualized. The sensitive fiber contribution of the glossopharyngeal nerve to the posterior one-third of the tongue was followed. In addition, the course of the lingual nerve and its branchings in the corpus towards the tip were demonstrated. Conclusion: We think that this overlooked stain technique should be considered when studying the complex innervations of structures, especially in reinnervation experiments.

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