Cemile Merve SEYMEN, İskender KAPLANOĞLU, Gülnur TAKE KAPLANOĞLU, Sinem YÜCEL, Bahriye Sirav ARAL

Kronik Cep Telefonu Radyasyonu ve Koruma Amaçlı Uygulanan Melatoninin Ovaryuma Etkisi

Amaç: Çalışmamızda cep telefonu radyasyonuna kronik maruziyetin ovaryumdokusu üzerinde meydana getireceği değişimler ve melatonin kullanımının budeğişimler üzerindeki olası koruyucu etkisinin ışık mikroskobik düzeydeincelenmesi amaçlandı.Yöntem: 24 adet Wistar albino cinsi dişi sıçan 4 gruba ayrılarak 90 günlükdeney süresince; kontrol grubuna hiçbir uygulama yapılmazken, 2. gruba hergün 30 dk 2100 MHz radyasyon uygulaması, 3. gruba her gün subkutanmelatonin uygulaması, 4. gruba ise radyasyon uygulamasından 40 dk öncesubkutan melatonin ve sonrasında 30 dk radyasyon uygulaması yapıldı.Deneybitiminde değerlendirme için ovaryum dokusundan alınan kesitlereHematoksilen-Eozin, TUNEL, PCNA boyaları uygulandı ve ışık mikroskobundaresimlendirildi. Yapılan PAS boyamalarında zona pellusida kalınlıkları ölçüldü.Ayrıca her kesitten folikül sayımı yapılarak, değişkenler istatistiksel olarakdeğerlendirildi.Bulgular: Hematoksilen-Eozin boyama ile yapılan incelemelerde radyasyongrubunda atreziye giden foliküllerin arttığı ve zona pellusida’nın yer yer kalınyer yer ince düzenlenim gösterdiği dikkati çekti. Radyasyon+Melatoningrubunda atreziye giden folikül sayısının azaldığı belirlendi. Bu bulgu, TUNELile desteklendi. PCNA immün boyamasında primordiyal, gelişen foliküller vekorpus luteum’da tutulumun kontrol grubuna karşın daha az sayıda hücredeolduğu izlendi.Sonuç: Sonuç olarak, melatonin enjeksiyonunun uygulanan melatoninin dozuya da uygulama süresine bağlı olarak radyasyonun neden olduğu yapısaldejenerasyonları kısmen engelleyebileceği kanısına varılmıştır.

Chronic Mobile Phone Radiation and the Possible Protective Effect of Melatonin on Ovary

Objective: We aim to investigate the possible protective effects of melatonin on ovary in the case of mobile phone radiation exposure by using light microscopic examinations. Methods: Twenty-four female Wistar albino rats were divided into 4 equal groups. Throughout in 90-day experiment, there was no application performed to control group. The second group was exposed to 2100 MHz radiation for 30 minutes. Subcutaneous melatonin was enjected to the third group. Subcutaneous melatonin enjection was applied 40 minutes before radiation and then radiation was exposed for 30 minutes to the fourth group. At the end of the experiment, ovary tissues were stained by HematoxylinEosin, TUNEL, PCNA and sections were photographed under light microscope. Zona pellusida thickness were measured in PAS stainings. Moreover, number of follicles were counted and all data were statistically analysed. Results: The number of atretic follicles were increased and zona pellucida were seen in thick and thin formations in radiation group according to Hematoxylin-Eosine staining. Atretic follicles were decreased in Radiation+Melatonin group, this finding was also supported by TUNEL. In PCNA immunstaining; the immunreactivity was seen in fewer cells compared to control group through primordial follicles, developing follicles and corpus luteum. Conclusion: In conclusion it was thought that melatonin injection could partially inhibited the structural degenerations caused by radiation depend on the dosage or application period.

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