Effect of taurine on liver xanthine oxidase activity and 3-nitrotyrosine level in endotoxemia
Amaç: Taurin, protein yapısına katılmayan, semi-esansiyel ve kükürt içeren bir β-amino asittir. Daha önce in vivove in vitro pek çok çalışmada taurinin hücre koruyucu etkilere sahip olduğu gösterilmiştir ve bu etkileri çoğunluklaantioksidan özelliğine bağlanmıştır. Bu çalışmada, taurinin endotoksemi esnasında hepatositlerdeki 3-nitrotirozinüretimi ve ksantin oksidaz aktivitesi üzerindeki etkilerinin araştırılması amaçlanmıştır. Yöntemler: 40 adet erkek Dunkin Hartley kobay, kontrol, taurin, endotoksemi ve taurin+endotoksemi olmak üzererastgele 4 gruba ayrılmıştır (n=10). Taurin grubuna 300 mg/kg ve tek doz taurin, endotoksemi grubuna 4 mg/kglipopolisakkarit, taurin+endotoksemi grubuna ise aynı dozlarda taurin ve lipopolisakkarit birlikte intraperitonealolarak uygulanmıştır. Uygulamadan 6 saat sonra, taurin ve endotoksin konsantrasyonları kanda en yüksek seviyeyeulaştığında, kobaylar anestezi altında feda edilmiş ve karaciğer dokuları uygun koşullarda alınmıştır. Dokulardaki3-nitrotirozin düzeyleri HPLC ile, ksantin oksidaz aktivitesi ise spektrofotometrik olarak ölçülmüştür.Bulgular: Lipopolisakkarit uygulaması, karaciğerde protein 3-nitrotirozin düzeyini ve ksantin oksidaz aktivitesini kontrol grubu ile karşılaştı rıldığında istatistiksel olarak anlamlı şekilde arttırmıştır. Taurin+endotoksemi grubunda ise, 3-nitrotirozin seviyesi ve ksantin oksidaz aktivitesi,endotoksemi grubuna göre belirgin azalma göstermiştir. Sonuç: Taurin, hepatositlerde proteinleri nitrasyon oluşumuna karşı korumuştur. Hem muhtemel peroksinitrit süpürücü etkisi ile, hem deksantin oksidaz aktivitesini azaltması yoluyla biyolojik bir antioksidan olarak etki gösterebilir. (Gazi Med J 2011; 22: 14-7)
Endotoksemide taurinin karaciğer ksantin oksidaz aktivitesi ve 3-nitrotirozin düzeylerine etkisi
Objective: Taurine, a semi-essential and sulphur-containing β-amino acid, is not incorporated into proteins. It hasbeen shown in many in vitro and in vivo studies to have cytoprotective effects, and these actions are often attrib uted to an antioxidant mechanism. The purpose of this study was to investigate the effect of taurine on 3-nitroty rosine production and xanthine oxidase activity in hepatocytes during endotoxemia. Methods: In this study, 40 adult male Dunkin Hartley guinea pig were randomly divided into four groups: con trol, taurine, endotoxemia and taurine plus endotoxemia (n=10). Animals were administered taurine (300 mg/kg,a single dose), lipopolysaccharide (4 mg/kg), or taurine plus lipopolysaccharide intraperitoneally. After six hours ofincubation, when the highest blood levels of taurine and endotoxin were attained, the animals were sacrificed andliver samples were collected. The amount of 3-nitrotyrosine was measured by HPLC and xanthine oxidase enzymeactivity was measured spectrophotometrically.Results: Lipopolysaccharide administration significantly increased protein 3-nitrotyrosine levels and xanthine oxi dase activity in hepatic tissue compared with the control group (p
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- Yayın Aralığı: Yılda 4 Sayı
- Yayıncı: Gazi Üniversitesi Tıp Fakültesi
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