Hakan ERİNÇ, İ. Hakkı İŞLER, Fatma SALUR

LİNOLEİK ASİDİN SAFLAŞTIRILMASINDA DÜŞÜK SICAKLIK KRİSTALİZASYON YÖNTEMİNİN ETKİNLİĞİ

Bu çalışmada linoleik asidin saflaştırılması amacıyla düşük sıcaklık kristalizasyon yönteminde farklı çözücülerin ve sıcaklıkların etkinliği araştırılmıştır. Bu amaçla aspir yağı sabunlaştırıldıktan sonra asitlendirilerek serbest yağ asitleri elde edilmiş, farklı çözücüler (petrol eter, izooktan, hekzan, aseton, izopropil alkol, metanol ve etanol) ve farklı sıcaklık değerleri (-40, -55 ve -70°C) kullanılarak linoleik asidin saflaştırılması gerçekleştirilmiştir. Yapılan çalışma sonucunda, -70°C’de kristalleşmeyen fazın çözücü olarak aseton kullanımı ile %98.17 ±1.91 ve hekzan kullanımı ile %92.61 ±1.63 saflıkta linoleik asit içerdiği belirlenmiştir. Hekzan kullanımı ile verim %63.99 ±1.19 iken aseton kullanımında bu oran %47.60 ±1.38 olarak belirlenmiştir. Diğer çözücülerin ise aspir yağından düşük sıcaklık kristalizasyon yöntemi ile linoleik asidin saflaştırılmasında çok fazla etkin olmadığı belirlenmiştir.

EFFECTIVENESS OF LOW TEMPERATURE CRYSTALLIZATION METHOD FOR PURIFICATION OF LINOLEIC ACID

In this study, the efficiency of different solvents and temperatures was investigated in the low temperature crystallization method in order to purify linoleic acid. For this purpose, the safflower oil was saponified and then acidified to produce free fatty acids (FFA) solution. Purification of linoleic acids from FFA solution has been carried out by low temperature crystallization method. FFA solution in different solvents (petroleum ether, isooctane, hexane, acetone, isopropyl alcohol, methanol and ethanol) were crystallized at several temperatures (-40, -55 and -70°C). As a result of this study, it was determined that liquid phase contained 97.1 ±1.91 % linoleic acid using acetone and 92.61 ±1.63 % using hexane at -70°C. The yield was 63.99 ±1.19 % and 47.60 ±1.38 % with using hexane and acetone, respectively. Other solvents were not found to be very effective for the purification of linoleic acid by low temperature crystallization of safflower oil.

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