Ali Said DURMUŞ, Aydın SAĞLIYAN, Cihan GÜNAY, Esra KİRMAN, Mustafa KÖM, Yasin BAYKALIR

Ratlarda Deneysel Deri Yaralarının İyileşmesi ÜzerineAnkaferd Blood Stopper’ın Etkilerinin Değerlendirilmesi

Bu çalışmada, geleneksel Türk tıbbında hemostatik bir ajan olarak kullanılan folklorik tıbbi bitkiözütü Ankaferd Blood Stopper'ın (ABS) deneysel standart tam kalınlıktaki deri yaralarının iyileşmesiüzerine olan etkileri incelendi. Çalışmada toplam olarak 36 rat kullanıldı. Tüm ratların sırtbölgesinde 5 mm'lik punch biyopsi ile deri yaraları oluşturuldu. Ratlar her bir grupta 12 rat olacakşekilde 3 gruba ayrıldı. Grup 1’deki ratlara lokal olarak günde iki defa ABS solüsyonu, Grup 2’dekiratlara yine günde iki kez madecassol uygulanırken Grup 3’deki ratlara herhangi bir tedaviuygulanmadı. Çalışmanın 7. ve 14. günlerinde her bir grupta altışar rat ötenazi edilerek yaraalanlarının makroskopik ölçümleri yapıldı. Grup 1 ve 2’deki yara alanlarının Grup 3’e göre dahahızlı kapanmasına rağmen istatistiksel olarak fark görülmedi. Histopatolojik incelemeler için yarabölgesinde doku örnekleri alınarak epitelizasyon, granulasyon dokusu oluşumu ve yangısal hücreinfiltrasyonu değerlendirildi. Yapılan histopatolojik değerlendirmelerde gruplar arasında istatistikselolarak anlamlı fark bulundu. Sonuç olarak doğal bir ürün olan ABS’nin güvenli bir ilaç olduğu, yaraiyileşmesi üzerinde herhangi bir olumsuz etki yapmadığı ve tam kat deri yaralarında etkili vegüvenli bir şekilde kullanılabileceği kanısına varılmıştır. Ancak ABS’nin yara iyileşmesi üzerindekiklinik ve histopatolojik yararlarını ve olası olumsuz etkilerini değerlendirmek için ek çalışmalaraihtiyaç vardır.

Evaluation of the Effects of Ankaferd Blood Stopper on the Healing of Experimental Skin Wounds in Rats

In this study, the effects of Ankaferd Blood Stopper (ABS), a folkloric medicinal plant extract usedas a haemostatic agent in traditional Turkish medicine, on wound healing of experimental standardfull-thickness skin defects were analysed. A total of 36 rats were used in the study. Skin woundswere created by 5 mm punch biopsy knots on the back of all rats. The rats were divided into 3groups with 12 rats in each group. While the rats in group 1 were applied ABS solution twice a daylocally, and the rats in group 2 madecassol twice a day, no treatment was applied to the rats ingroup 3. On the 7th and 14th days of the study, six rats in each group were euthanized andmacroscopic measurements of the wound areas were made. The wound areas in group 1 and 2healed faster than group 3, but there was no significant difference. For histopathologicalexaminations, tissue samples were taken at the wound site and epithelization, granulation tissueformation and inflammatory cell infiltration were evaluated. A statistically significant difference wasfound between the groups in the histopathological evaluations. As a result, it has been concludedthat ABS, which is a natural product, is a safe drug, does not have any negative effect on woundhealing and can be used effectively and safely in full-thickness skin wounds. However, additionalstudies are needed to evaluate the clinical and histopathological benefits and possible negativeeffects of ABS on wound healing.

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