İlter KUŞ, İsmail ZARARSIZ, Murat ÖGETÜRK, H. Ramazan YILMAZ, Mustafa SARSILMAZ

Deneysel Formaldehit Toksisitesinde Testis SOD, GSH-Px, MDA Düzeyleri ve ω-3 Yağ Asitlerinin Koruyucu Etkisi

Amaç: Biyokimyasal düzeyde yapmış olduğumuz bu çalışmada, formaldehit toksisitesinin testisler üzerindeki etkilerinin incelenmesi amaçlandı. Bunun yanı sıra, bu toksisiteye karşı omega-3 yağ asitlerinin olası antioksidan etkileri de değerlendirildi.. Gereç ve Yöntem: Çalışmamızda, toplam 21 adet Wistar-Albino cinsi erişkin erkek sıçan üç eşit gruba ayrıldı. I. gruptaki sıçanlar kontrol olarak kullanıldı. II. gruptaki hayvanlara gün aşırı olarak ve de intraperitoneal yolla formaldehit enjekte edildi. Son gruptaki sıçanlara ise formaldehit enjeksiyonu ile birlikte intragastrik gavaj yoluyla ve günlük olarak omega-3 yağ asiti (Marincap kapsül®) verildi. 14 günlük uygulama periyodu sonunda bütün hayvanlar dekapite edilerek testisleri çıkartıldı. Daha sonra testis doku örneklerine ait süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) enzim aktivasyonları ile malondialdehit (MDA) düzeyleri spektrofotometrik olarak belirlendi. Bulgular: Sadece formaldehit verilen gruba ait biyokimyasal değerler incelendiğinde, testis SOD ve GSH-Px enzim aktivasyonlarının kontrol grubuna göre istatistiksel olarak anlamlı bir biçimde azalmış olduğu, MDA düzeylerinin ise yine anlamlı bir şekilde yükseldiği görüldü. Diğer yandan, formaldehit uygulaması ile birlikte omega-3 yağ asiti verilen sıçanlarda, testis doku örneklerine ait SOD ve GSH-Px enzim değerlerinin arttığı, MDA seviyelerinin de azaldığı tespit edildi. Sonuç: Sıçanlar üzerinde gerçekleştirmiş olduğumuz bu çalışmada, deneysel formaldehit toksisitesi sonucu testislerde meydana gelen oksidatif doku hasarının omega-3 yağ asitleri tarafından engellendiği biyokimyasal olarak ortaya kondu.©2008, Fırat Üniversitesi, Tıp Fakültesi

Anahtar Kelimeler:

Formaldehit, testis, oksidatif doku hasarı, Omega-3 yağ asitleri

Testicular SOD, GSH-Px and MDA Levels in Experimental Toxicity of Formaldehyde and Protective Effect of ω-3 Fatty Acids

Objective: In this study, it was aimed to investigate the effects of formaldehyde toxicity on the testes at biochemical level. Potential antioxidant effects of ω-3 fatty acids against these toxic effects were also evaluated. Materials and Methods: In our study, a total of 21 adult male Wistar rats were divided into three equal groups. Rats in group I were used as control. Rats in group II were injected intraperitoneally with formaldehyde every other day while the other ones received ω-3 fatty acids daily via intragastric gavage (Marincap capsule®) with injections of formaldehyde. At the end of 14-days experimental period, all animals were killed by decapitation and their testes were removed. Afterwards, the activites of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the level of malondialdehyde (MDA) in testicular tissue specimens were determined spectrophotometrically. Results: Biochemical values of group-treated formaldehyde alone showed that the enzymatic activities of testicular SOD and GSH-Px were significantly decreased compared to control values, while testicular MDA levels were significantly increased. On the other hand, it was found that there were increases in SOD and GSH-Px enzyme activities, and decreases in MDA levels in testis specimens of rats administered ω-3 fatty acids with exposure of formaldehyde. Conclusions: In this study performed on rats, it was determined biochemically that oxidative tissue damage in testes resulting from experimental toxicity of formaldehyde was prevented by omega-3 fatty acids.. ©2008, , Firat University, Medical Faculty

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Kabul Tarihi:21.07.2007