Ayşe Belin ÖZER, Dilara KAMAN, Ömer Lütfi ERHAN, Songül ÖZER

EGCg İlavesi Sevofluran Uygulanan Ratların Böbreklerinde Oksidan ve Antioksidan Durumunu Düzenler

Amaç: Ratlarda böbrek dokusu üzerinde oksidan ve antioksidan durumu değerlendirerek sevofluran anestezisi ve epigallocatechin 3- gallate (EGCg)'ın muhtemel etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Ratlar 3 gruba ayrılarak çalışıldı. Grup 1 (n:7): Sevofluran, %3 (v/v) hava/O2 karışımı ile uygulandı. Grup 2 (n:7): Sevofluran + EGCg uygulandı. Kontrol grubu için ise yine 7 tane rat seçildi. Malondialdehid (MDA), nitrik oksit (NO), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) böbrek dokusunda çalışıldı. Ayrıca histopatolojik değişiklikler değerlendirildi. Bulgular: Grup 1'de MDA, NO, SOD ve GSH-Px aktiviteleri artmışken, CAT aktivitesi sevofluran uygulananlarda azalmıştır. EGCg'nin uygulaması (grup 2) MDA, SOD ve GSH-Px aktivitelerini azaltırken, NO ve CAT aktiviteleri grup 1 ile karşılaştırıldığında artmış olarak bulunmuştur. Histopatolojide sevofluran uygulanan grupta böbrek dokusunda orta derecede kortikal nekroz ve şiddetli dejenerasyonla karakterize değişimlerin oluştuğu saptandı. Sevofluran ile birlikte EGCg uygulamasının ise dejenerasyonu azalttığı bulunmuştur. Sonuç: Lipid peroksidasyonu ve antioksidan enzim düzeyleri sevofluran uygulamasını takiben fazla miktarda artmıştır. Ancak, EGCg uygulaması böbrek dokusunu önemli ölçüde korumaktadır. ©2008, Fırat Üniversitesi, Tıp Fakültesi

Anahtar Kelimeler:

EGCg, Sevofluran, oksidan-antioksidan sistem

EGCg Supplementation Improves Oxidant and Antioxidant Status in Kidney of Rats Exposed to Sevoflurane

Objectives: To investigate the possible effects of sevoflurane anesthesia and epigallocatechin 3- gallate (EGCg) on kidney tissue by evaluating the oxidant and antioxidant status in rats. Materials and Methods: Tree groups of animals were studied. Sevoflurane 3% (v/v) in air/O2 were administered to animals in group 1 (n = 7) and sevoflurane plus EGCg was administered in group 2 (n = 7). Seven animals were allocated to control group (group 3). Malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in kidney tissue were studied. Histopathological changes were also assessed. Results: In group I, MDA, NO, SOD and GSH-Px activities were increased while decreased CAT activities occurred when sevoflurane was exposured compared with controls. Supplementation of EGCg (group 2) decreased the MDA (P

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Gonsowski CT, Laster MJ, Eger EI, Ferrell LD, Kerschmann RL. Toxicity of compound A in rats: effect of increasing duration of administration. Anesthesiology 1994; 80: 556-565.
Higuchi H, Arimura S, Sumikura H, Satoh T, Kanno M. Urine concentrating ability after prolonged sevoflurane anaesthesia. Br J Anaesth 1994; 73: 239-240.
Bito H, Ikeda K. Plasma inorganic fluoride and intracircuit degradation product concentrations in long-duration, low-flow sevoflurane anesthesia. Anesth Analg 1994; 79: 946-951.
Morio M, Fujii K, Satoh N, et al. Reaction of sevoflurane and its degradation products with soda lime. Anesthesiology 1992; 77: 1155-64.
Kandel L, Laster MJ, Eger EI, Kerschmann RL, Martin J. Nephrotoxicity in rats undergoing a one-hour exposure to compound A. Anesth Analg 1995; 81: 559-63.
Martin JL, Laster MJ, Kandel L, et al. Metabolism of compound A by renal cysteine-S-conjugate beta-lyase is not the mechanism of compound A-induced renal injury in the rat. Anesth Analg 1996; 82: 770-774
Eger EI, Gong D, Koblin DD, et al. Dose-related biochemical markers of renal injury after sevoflurane versus desflurane anesthesia in volunteers. Anesth Analg 1997; 85: 1154-1163.
Eger EI, Koblin DD, Bowland T, et al. Nephrotoxicity of sevoflurane versus desflurane anesthesia in volunteers. Anesth Analg 1997; 84: 160-168.
Mitscher LA, Jung M, Shankel D, et al. Chemoprotection: a review of the potential therapeutic antioxidant properties of green tea (Camellia sinensis) and certain of its constituents. Med. Res. Rev. 1997; 17:327-365
Rice-Evans C. Plant polyphenols: free radical scavengers or chain-breaking antioxidants? Biochem Soc Symp. 1995; 61: 103- 116.
Kuo SM, Leavitt PS, Lin CP. Dietary flavonoids interact with trace metals and affect metallothionein level in human intestinal cells. Biol. Trace Element Res. 1998; 62: 135-53.
Yoshino M, Murakami K. Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anal. Biochem. 1998; 257: 40-44.
Lowry O, Rosenbraugh N, Farr L,Randall R. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951; 182, 265–275
Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990; 36: 1440-1443.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Analytical Biochemistry. 1979; 95: 351-358.
Durak I, Yurtarslani Z, Canbolat O, Akyol O. A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta; 1993; 214: 103-104.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab & Clin Med 1967; 70: 158-169.
Aebi H. Catalase In: Bergmeyer U, ed. Methods of enzymatic analysis. New York and London: Academic Press; 1974; 673- 677.
McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000; 108: 652–659.
Matés JM, Pérez-Gómez C, Nşñez de Castro I. Antioxidant enzymes and human diseases. Clin Biochem 1999; 32: 595–603.
Sivaci R, Kahraman A, Serteser M, Sahin DA, Dilek ON. Cytotoxic effects of volatile anesthetics with free radicals undergoing laparoscopic surgery. Clin Biochem. 2006; 39: 293- 298.
Lorenz M, Wessler S, Follmann E, et al. Constituent of green tea, epigallocatechin-3-gallate, activates endothelial nitric oxide synthase by a phosphatidylinositol-3-OH-kinase- cAMP- dependent protein kinase-, and Akt-dependent pathway and leads to endothelial-dependent vasorelaxation. J Biol Chem 2004; 13: 6190-6195.
Frei B., Higdon J.V. Antioxidant activity of tea polyphenols in vivo: evidence from animal studies, J. Nutr. 2003;133: 3275– 3284
Dikmen B, Unal Y, Pampal HK. Effects of repeated desflurane and sevoflurane anesthesia on enzymatic free radical scavanger system. Molecular and Cellular Biochemistry, 2007; 294: 31-36.
Kabul Tarihi: 10.08.2007