Sebahat GÜVEN, Mustafa PEHLİVAN, Mehmet YILMAZ, Vahap OKAN, Sacide PEHLİVAN

Akut lösemilerde MIF Genindeki polimorfizmlerin önemi ve febril nötropenîk ataklara etkisi

Bu çalışmanın amacı; Akut Lösemiler (AL)'de Makrofaj Migrasyon inhibitor Faktörü (MIF) geninin -173 bölgesine ait polimorfizmleri araştırmak ve kemoterapi sonrası gelişen febril nötropenik (FN) ataklara etkisini değerlendirmektir. Akut Myeloblastik Lösemi (AML) ve Akut Lenfoid Lösemi (ALL) tanısıyla tedavi gören 48 hasta ile 53 sağlıklı kontrolden izole edilen DNA örneklerinde MIF geni -173 bölgesine ait tek nükleotid polimorfizmleri, PCR-RFLP yöntemi ile araştırılarak, G ye C allel ve genotip dağılımları hasta ile kontrol grupları arasında karşılaştırmıştır. Analizler sonucunda GG genotipini taşıyan hastaların oranı %70.9, GC'yi taşıyanların oranı %29.1 ye CC'yi taşıyanların oranı %0 olarak saptanmıştır. Hasta ve kontrol grubunda genotip ye allel frekansları açısından anlamlı bir farklılık ile Hardy-Weinberg dengesinden bir sapma gözlenmemiştir. AL'lerde elde edilen MIF pozitifliği ile infeksiyonların dağılımı,'etkenleri, ateşli gün süresi ve nötropeni süresi arasındaki ilişki değerlendirildiğinde; GC allel grubunda, infeksiyonların etkenleri, nötropeni (0.05). Sonuç olarak; Fungal - pnömoni, GC aleli taşıyan grupta, GG aleli taşıyan gruba göre (%22/ll,5) daha sık izlenmiş olup çalışma grubunun' genişletilerek tekrarlanması durumunda anlamlı bir ilişki saptanabileceği düşünülmüştür.

Anahtar Kelimeler:

Makrofaj Migrasyon Ýnhibitör Faktör Geni, -173G/C polimorfizmi, PCR, Akut Lösemi, DNA

Impotance of polymorphisms of the MIF gene at acute leukemia and its effect on febrile neutropenic attacks

The objective of this study is to research polymorphisms in MIF ' gene -173 site in variable haemotological malignities and evaluate its effects on febrile neutropenic attacks after chemotherapy in acute leukemia. Single nucleotide polymorphisms (SNPs) at -173 site of the MIF gene was analysed with the PCR-RFLP method (restriction endonudease Alu I) with isolated DNA samples from 48 patients who were treated for ALL and AML and 53 healthy controls, and G and C allel frequencies were compared statistically between patients and healthy controls groups. As a result of the analyzis, it was observed that the rate of patients with GG genotypes was 70.9%, with GC genotypes 29.1%, and with CC genotypes 0%.,Association is evaluated between MIF pozitivities in acute leukemias and distribution of infections, its factors, duration of feveraldays and neutropenia. Within the GC allel group; no significant differences were observed (p>0.05) in infection factors, duration of neutropenia (

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