Elif VAR ODABAŞ, Semra ÜNAL YILDIRIM, Oğuzhan GÜNDÜZ, Berna ALEMDAĞ, Deniz Mukaddes TÜRET, Ömer Birkan AĞRALI

Bakteriyel Selüloz Tabanlı Hidrojel Taşıyıcı ile Topikal Uygulanan Tideglusib’in Sıçanlarda Oluşturulan Palatinal Yaranın İyileşmesi Üzerine Etkisinin Makroskobik Değerlendirmesi: Bir Öncül Çalışma

Amaç: Bu çalışmanın amacı, bakteriyel selüloz (BS) tabanlı hidrojel taşıyıcı ile topikal olarak uygulanan tideglusib (Td)'nin sıçanlarda oluşturulan palatinal yaranın iyileşmesi üzerindeki erken etkisini makroskobik olarak değerlendirmektir. Gereç ve Yöntemler: Bu çalışmada deney hayvanı olarak; ortalama 3 aylık, ağırlıkları 250-300 gr arasında değişen, 12 adet genç erişkin erkek Sprague Dawley sıçan kullanıldı. Sıçanların palatinal mukozalarının ortasında 4 mm çapında mukozal defekt oluşturuldu. Üç hayvan, defekt oluşturulduktan hemen sonra sakrifiye edildi ve başlangıç grubunu oluşturdu. Diğer 9 hayvan, pasif kontrol, sadece taşıyıcı uygulanan (hidrojel) ve taşıyıcı içinde Td uygulanan (Td+Hidrojel) olmak üzere rastgele üç gruba ayrıldı. Bu üç gruptaki hayvanlar 7. günde sakrifiye edildi. Ortalama yara yüzey alanı fotoğraf üzerinden ölçülerek makroskobik olarak değerlendirildi. Bulgular: Gruplar arası karşılaştırmada yara yüzey alanında istatistiksel olarak anlamlı farklılık gözlendi (p=0,033). Gruplar arası ikili karşılaştırmalarda sadece başlangıç grubu ile Td+Hidrojel grubu arasında istatistiksel olarak anlamlı bir fark bulundu (p=0,028). Sonuç: BS tabanlı hidrojel ile topikal olarak uygulanan Td’nin, sıçanların palatal mukozal yara iyileşmesi üzerinde olumlu etkilere sahip olduğu makroskobik olarak gösterilmiştir.

Anahtar Kelimeler:

yara iyileşmesi, palatinal mukozal iyileşme, yara örtücü, tideglusib, wnt sinyal yolu

Macroscopic Evaluation of the Effect of Topically Applied Tideglusib with Bacterial Cellulose-Based Hydrogel Carrier on Healing of Palatal Wound in Rats: A Preliminary Study

Objectives: The aim of this study is to macroscopically evaluate the early effect of topically applied bacterial cellulose (BC) based hydrogel carrier including tideglusib (Td) on palatal wound in rats. Materials and Methods: The study group consisted of 3 months old male, 12 Sprague Dawley rats, each weighing an average of 250-300 gr. Three animals were instantly sacrificed and represented the baseline group at time 0 (initial group). The remaining nine were divided into three groups at random: empty group, hydrogel group, Td+hydrogel group. Mean wound surface area was measured photographically. Results: In the intergroup comparison, a statistically significant difference was observed in the wound surface area (p=0.033). In the pairwise comparisons between the groups, a statistically significant difference was found only between the initial group and the Td+Hydrogel group (p=0,028). Conclusions: Td topically applied with BC based hydrogel has positive effects on palatal mucosal wound healing of rats.

Keywords:

wound healing, palatal mucosal healing, wound dressing, tideglusib, wnt signaling pathway,

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