Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori
Böcek yağ dokusu, lipit, karbohidrat, aminoasit ve nitrojen metabolizmalarına katılan ve protein sentezinde rol alan en büyük biyosentetik depo organıdır. Bunun yanı sıra, vitellogenin gibi yumurta depo proteinlerinin öncülerini sentezleyerek aktif olarak vitellogenezde ve ovaryum gelişiminde rol alır. Bombyx mori’de (L.)’te metamorfoz süresince, yağ doku, hemolenf ve ovaryum arasında meydana gelen sürekli madde değişimi ekdisteroidler ve juvenile hormonun kontrolü altında gerçekleşir. Elektroforetik olarak belirlenen temel hemolenf proteinleri şu şekilde gruplandırılmıştır: depo proteinleri (72 kDa ve 76 kDa), 30K polipeptidleri, ApoLp-I (230- 250 kDa), yumurta spesifik proteini (/2 kDa ve 64 kDa) ve vitellogenin (178 kDa). Çalışmamızın sonucunda metamorfoz süresince yağ doku proteinlerinin nitelik ve niceliğindeki değişimlerin ovaryum ve yumurta gelişimi üzerinde doğrudan etkiye sahip olduğu sonucuna ulaşılmıştır.
Anahtar Kelimeler:
Bombyx mori, hemolenf proteinleri, yağ doku proteinleri, yumurta depo proteinleri,
The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, aminoacid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis andovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specificmanner. The continual substance exchange among fat body, hemolymph and ovary is controlled byhormones, ecdysteroids and juvenile hormone, during metamorphosis ofBombyx mori (L.). The aimof the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.
Keywords:
Bombyx mori, hemolymph proteins, fat body proteins, yolk proteins, SDS-PAGE.,
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- ISSN: 2602-2575
- Yayın Aralığı: Yılda 2 Sayı
- Başlangıç: 1940
- Yayıncı: İstanbul Üniversitesi Yayınevi
Sayıdaki Diğer Makaleler
Semra SEKME, Neşe ATACI, İnci ARISAN
Neslihan BALKIS, Nüket SİVRİ, Linda FRAİM, Muharrem BALCI, Turgay DURMUŞ, Atakan SUKATAR
Esen POYRAZ, Ebru GÖNCÜ, Osman PARLAK
Güneydoğu Anadolu’dan Suncus etruscus (Savi, 1822) (Mammalia: Soricidae) Kayıtları
Ayten Kimiran - Erdem1*, Elif Özlem Arslan- Aydoğdu1, Sevan Gürün2