Farklı diş macunlarının antibakteriyel etkilerinin in vitro olarak incelenmesi

Bu in vitro çalışmada 8 farklı diş macununun Streptococcus mutans, Enterococcus faecalis, Bacillus subtilis, Lactobacillus casei, Staphylococus aureus üzerine antibakteriyel etkileri Agar Difüzyon Testi ile incelendi. Gereç ve yöntem Farklı özellikteki diş macunlarının antibakteriyel etkilerini incelemek için, agar plakalarının üzerine 5 mm genişliğinde yuvalar açıldı. Her bir diş macunu bu yuvalara yerleştirildi. Yuvalardan bir tanesi kontrol grubu olması amacı ile boş bırakıldı. 24 saat sonra test edilen mikroorganizmalara karşı oluşan inhibisyon alanları ölçülerek değerlendirmeler yapıldı. Sonuçların istatistiksel analizi için Kruskal Wallis and Mann Whitney U Testi kullanıldı. Sonuçlar Yirmi dört saat sonra yapılan gözlemler test edilen her diş macununun antibakteriyel etkilerinin olduğunu fakat özellikle Streptococcus mutans için diş macunları arasında istatistiksel fark oluşmadığını gösterdi P>0,05 .

IN VITRO ANTIBACTERIAL ACTIVITY OF DIFFERENT TOOTHPASTES

In this in vitro study eight toothpastes were tested for their antimicrobial activity against four oral pathogens namely, Streptococcus mutans, Enterococcus faecalis, Bacillus subtilis, Lactobacillus casei, Staphylococus aureus by well Agar Diffusion Test. Method and materıals For the evaluation of antibacterial properties of materials, 9 holes prepared by 5 mm diameter on each agar plate. Different 8 toothpastes insert in this holes but one of them lived empty for control. Inhibition zones of against the test organism measured after 24 hours. Kruskal Wallis and Mann Whitney U Test were used for evaluate of the findings by statistically. Results According to 24 h results all tested toothpastes have antimicrobial activity on tested organism type but there was not statistically differences on Streptococcus mutans P>0,05 .

___

  • 1. Pannuti CM, Mattos JP, Ranoya PN, Jesus AM, Lotufo RF & Romito, GA. Clinical effect of a herbal dentifrice on the control of plaque and gingivitis: a double-blind study. Pesqui Odontol Bras ; 2003, 17: 314-8.
  • 2. Degiam ZD. An In Vitro Antimicrobial Activity Of Six Commercial Toothpastes. Thi-Qar Medical Journal: 2010,4(4): 127-33.
  • 3. Fine DH, Furgang D, Markowitz K, Sreenivasan PK, Klimpel, K.& De Vizio W. The antimicrobial effect of a triclosan/copolymer dentifrice on oral microorganisms in vivo. J Am Dent Assoc. 2006,137: 1406-13.
  • 4. Moran J. Addy M.& Newcombe R. The antibacterial effect of toothpastes on the salivary flora. J Clin Periodontol, 1988, 15: 193-9.
  • 5. Ozak, F, Pannuti CM, Imbronito AV, Pessotti W, Saraiva, L, de Freitas ,NM. Efficacy of a herbal toothpaste on patients with established gingivitis a randomized controlled trial. Braz Oral Res. 2006, 20, 172-7.
  • 6. Çakir FY, Gürgan S, Attar N. Çürük Mikrobiyolojisi. H Diş Hek Fak Derg 2010,:34, 3, 78-91.
  • 7. Bader JD, Shugars DA, Bonito AJ. A systematic review of selected caries prevention and management methods. Community Dent Oral Epidemiol 2001; 29: 399–411.
  • 8. Roberson TM, Heymann OH, Swift EJ. Sturdevant’s Art and Science of Operative Dentistry, Gürgan S, Yalcin Cakir F, 3. Bölüm: Karyoloji: Lezyon, Etyoloji, Önleme ve Kontrol (Cariology: The Lesion, Etiology, Prevention and Control), Ankara: Güneş Tıp Kitabevleri; 2010, 67- 134.
  • 9. Tazegül S, Koçak MM, Topuz Ö, Özcan S, Çekiç AA, Erten H. Üç Farklı Solüsyonun Streptococcus mutans ve Enterococcus faecalis Üzerine Antimikrobiyal Etkinliklerinin Değerlendirilmesi EÜ Dişhek Fak Derg 2006; 27: 153-8.
  • 10. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003; 36: 1-11.
  • 11. Love RM. Enterococcus faecalis-a mechanism for its role in endodontic failure. Int Endod J 2001; 34: 399- 405.
  • 12. Lee, SS.; Zhang, W& Li ,Y. The antimicrobial potential of 14 natural herbal dentifrices: results of an in vitro diffusion method study. J. Am Dent Assoc. 2004. 135,8: 1133-41.
  • 13. Kolahi J, Soolari A. Rinsing with chlorhexidine gluconate solution after brushing and flossing teeth. A systematic review of effectiveness. Quinttessence Int. 2006; 37(8): 605- 612.
  • 14.Barkvoll P, Rølla G, Svendsen K. Interaction between chlorhexidine digluconate and sodium lauryl sulfate in vivo. J Clin Periodontol. 1989 Oct;16(9):593-5.
  • 15. Motisuki C, Monti LL, Spolidorio DMP, Santos-Pinto L. Influence of sample type and collection method on Streptococcus mutans and Lactobacillus counts in the oral cavity. Arch Oral Biol 2005; 50: 341-345.
  • 16. Davies RM, Ellwood RP. & Davies GM. The effectiveness of a toothpaste containing triclosan and polyvinyl-methyl ether maleic acid copolymer in improving plaque control and gingival health: a systematic review. J Clin Periodontol. 2004, 31: 1029-33.
  • 17. Sofrata A, Santangelo EM, Azeem M, Borg-Karlson AK, Gustafsson A, Pütsep KBenzyl isothiocyanate, a major component from the roots of Salvadora persica is highly active against Gram-negative bacteria. PLoS One. 2011; 6(8).
  • 18. Olsson B. Efficiency of traditional chewing sticks in oral hygiene programs among Ethiopian schoolchildren Community Dent. Oral Epidemiol. 1978, 6 ,3 105–109
  • 19. M Norton, M Addy. Chewing sticks versus toothbrushes in West Africa. A pilot study. Clin Prev Dent. 1989, 11, 11–3.
  • 20. M Moustafa, A Abd, F Abo. Reduced plaque formation by miswak-based mouthwash Egypt Dent J., 1987, 33,4, 375–384.
  • 21.B Danielsen, V Baelum, F Manji, O Fejerskov. Chewing sticks, toothpaste, and plaque removal, Acta. Odontol. Scand. 1989, 47 ,2, 121– 125.
  • 22. Sadeghi M, Assar S. An in vitro antimicrobial activity of ten Iranian made toothpastes, Dent Res J 2009;6,2:87-92.
  • 23. AbdelRahman HF, Skaug MPN, Francis GW. In vitro antimicrobial effects of crude miswak extracts on oral pathogens. Saudi Dental Journal, 2002, 14, 1.
  • 24. Mohammed SG, comparative study of in vitro antibacterial activity of miswak extracts and different toothpastes. American Journal of Agricultural and Biological Sciences, 2013, 8,1, 82-88.
  • 25. Prasanth M, Antimicrobial Efficacy of Different Toothpastes and Mouthrinses: An In Vitro Study Dent Res J 2011; 8,2: 85-94.
  • 26. Guggenheim B, Lutz F, Schmid R: Caries and plaque itihibition in rats by five topically applied dentifrices. Eur J Oral Sci 1997: l05: 258-263.
  • 27. Saxer U, Jaschouz V, Ley F. The effect of Parodontax dentifrice on gingival bleeding. The Journal of Clinical Dentistry 1994: 5(2):63-64.
  • 28. Mullally BH, James JA, Coulter WA, Linden GJ. The efficacy of a herbal-based toothpaste on the control of plaque and gingivitis. J Clin Periodontol. 1995 Sep;22(9):686-9.