2010

Yumurtaya verilen bisfenol a ’nın, tavuklarda timusun gelişimi ve perifer kan alfa naftil asetat esteraz pozitif lenfosit oranı üzerindeki etkilerinin histolojik ve enzim histokimyasal yöntemlerle belirlenmesi

Amaç: Bu çalışmanın amacı yumurtaya verilen Bisfenol A’nın, tavuklarda timusun gelişimi ve perifer kan alfa naftil asetat esteraz (ANAE) pozitif lenfosit oranı üzerindeki etkilerininhistolojik ve enzimhistokimyasal metodlar kullanarak belirlemektir.Gereç ve Yöntem: Bu amaçla Isa Brown ırkı yumurtacı tavuklara ait 310 adet her biri 50-55 g ağırlığında olan döllü yumurta kontrol, taşıyıcı kontrol, 50, 100, 250 μg/yumurtaBPA olmak üzere 5 gruba ayrıldı. Test solüsyonları inkübasyondan önce yumurta sarısına enjekte edildi. Kuluçkanın 13, 18 ve 21. günlerinde her gruptan 10’ar adet yumurta açıldıve elde edilen embriyolardan kan ve timus dokusu örnekleri alındı.Bulgular: Kuluçkanın 13. 18. ve 21. günlerinde BPA uygulanan gruplarda kontrol grubuna göre timus dokusunun embriyonik gelişiminin geri olduğu tespit edildi. Aynı zamandaBPA verilen gruplarda lenfoid dokunun daha az hücre yoğunluğuna sahip olduğu ve ANAE pozitif lenfositlerin sayıca azaldığı dikkati çekti. Perifer kan ANAE pozitif lenfosit oranlarının da kontrol gruplarına göre BPA verilen gruplarda önemli oranda düştüğü tespit edildi (p

The determination of effects of in ovo administrated bisphenol a on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzymehistochemical methods in chicken

Aim: The aim of this study is to the determination of effects of in ovo administrated Bisphenol A on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzyme histochemical methods in chicken.Materials and Methods: For this purpose, 310 fertile eggs, weighed 50-50 g, of Isa Brown laying parent stock were divided into 5 groups as control, vehicle- control , 50 100, and 250 μg/egg BPA. Test solutions were injected into yolk before incubation. At the 13th, 18th and 21st days of incubation, 10 eggs were opened from each group and blood and thymus tissue samples were taken from the obtained embryos. Results:On the 13th, 18th and 21st days of incubation, BPAtreated groups were found to be retarded embryonic development of thymus tissue compared to the control group. At the same time, in BPA-treated groups, lymphoid tissue had less cell density and the number of ANAE positive lymphocytes decreased. The percentage of peripheral blood ANAE positive lymphocytes was significantly lower in the BPA-treated groups than in the control groups (p

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