H. Hüseyin HADİMLİ, Aslı SAKMANOĞLU, Osman ERGANİŞ, Kürşat KAV, Zafer SAYIN

Subklinik mastitisli süt ineklerinden izole edilen koagulaz negatif stafilokokların identifikasyonu ve antibiyotiklere duyarlılıkları

Amaç: Bu çalışmada subklinik mastitisli süt ineklerinden izole edilen koagulaz negatif stafilokokların (KNS) identifikasyonu ve antibiyotiklere duyarlılıklarının belirlenmesi amaçlandı.Gereç ve Yöntem: Subklinik mastitisli inek sütlerinden izole edilen toplam 286 KNS izolatı koloni morfolojisi, hemoliz ve biyokimyasal özelliklerine göre identifiye edildi.Bulgular: İzolatların 40'ı S. simulans (%14.98), 31'i S. epidermidis (%11.15), 54'ü S. chromogenes (%19,42), 31'i S. xylosus (%11.15), 22'si S. caprae (%7.91), 19'u S. warneri (%6.83), 18'i S. haemolyticus (%6.47), 13'ü S. cheiferi subsp. coagulans (%4.67), 10'u S. cohneii subsp. urealyticus (%3.59), 10'u S. saprophyticus (%3.59), 6'sı S. gallinarum (%2.15), 7'si S. hominis (%2.51), 4'ü S. hyicus (%1.43), 2'si S. cheiferi subsp. cheiferi (%0.71), 9'u S. lentus (%3.23) ve 2'si S. sciuri (%0.71) olarak identifiye edildi. KNS'ların farklı antibiyotiklere duyarlılıklarının değişken olduğu gözlendi.Öneri: Bu çalışma, subklinik mastitisli inek sütlerinden izole edilen KNS'ların tam identifikasyonla

Identification and antibiotic susceptibility of coagulase negative staphylococci isolated from dairy cows with subclinical mastitis

Aim: This study was aimed to identify of coagulase negative staphylococci (CNS) isolated from bovine milk samples with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics.Materials and Methods: Totally, 286 CNS isolates from milk samples were identified according to colony morphology, haemolysis and biochemical properties.Results: Out of isolates, 40 (14.38%) were identified as S. simulans, 31 (11.15%) S. epidermidis, 54 (19.42%) S. chromogenes, 31 (11.15%) S. xylosus, 22 (7.91%) S. caprae, 19 (6.83%) S. warneri, 18 (6.47%) S. haemolyticus, 13 (4.67%) S. cheiferi subsp. coagulans, 10 (3.59%) S. cohneii subsp. urealyticus, 10 (3.59%) S. saprophyticus, 6 (2.15%) S. gallinarum, 7 (2.51%) S. hominis, 4 (1.43%) S. hyicus, 2 (0.71%) S. cheiferi subsp. cheiferi, 9 (3.23%) S. lentus and 2 (0.71%) S. sciuri. The susceptibilities of CNS were determined to be variable to different antimicrobial agents.Conclusions: This study was shown that identification of CNS isolated from dairy cows with subclinical mastitis should be strictly performed.

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