Natural ovine pulmonary adenocarcinoma in an Egyptian sheep farm

Ovine pulmonar adenokarsinoma koyunlarda retrovirüsün neden olduğu akciğer kanseridir. Mevcut vaka raporunun amacı Mısır'da bir koyun çiftliğinde hastalığın histopatalojik ve klinik tanımı yapmaktır. Çiftlikteki 4 adeti ölü ve 5 adeti hasta genç koyuna klinik, makroskobik ve histopatalojik değerlendirmeler yapıldı. Histopatalojik incelemeler için akciğer, mediastinal lenf yumruları, karaciğer, kalp, böbrek ve beyin dokusu örnekleri alındı. Örnekler %10 formalde tespit edildi ve ışık mikroskopta incelendi. İncelene hayvanlarda ağır kilo kaybı ve burunda mokuid akıntı gözlendi. Nekropside akciğer çok sayıda grimsi köpüklü sıvı ve pleural boşluk mukois sıvı birikimi gözlendi. Histopatalojik incelemede çok sayıda alveolar epitek kökenli kapsülsüz neoplastik odaklar tespit edildi. Neoplastik odak kübik ve sütun hücrelerden oluşmaktaydı. Bunlar düzenli papillar ve asiner şekilde belirlendi. Proliferatif hücreler küçük damar hücreleri ve fibroblast içeren yumuşak doku ile desteklenmiş olarak belirlendi. Neoplastic odaklarla ilişkili alveolar boşluklarda çok sayıda şişkin makrofaj varlığı belirgindi. Sonuç olarak ovine pulmonar adenokarsinomalar için kübik ve uzun hücrelerle kaplı alveolar epitelin transformasyonu ve burundan mukoid sıvı akıntısı patognomiktir.

Mısır'da bir koyun çiftliğinde ovine pulmoner adenokarsinoma

Ovine pulmonary adenocarcinoma is a naturally occurring retrovirus-induced contagious lung neoplasm in sheep. The current report aims to describe clinical and histopathological characteristics of the disease in an Egyptian sheep farm. In which, 4 dead and 5 sick young sheep were subjected to clinical, gross and histopathological examinations. For histopathological examination, tissue specimens were taken from lungs, mediastinal lymph nodes, liver, heart, kidneys and brain, fixed in 10% neutral buffered formalin and processed for light microscopy. Examined animals showed release of copious amount of mucoid fluid from their nostrils and marked loss of body weight. At necropsy, there were many solid grayish nodules exuding frothy fluid in the lungs and accumulation of mucoid fluid in the pleural cavity. Histopathological examination revealed presence of many nonencapsulated neoplastic foci originating from the alveolar epithelial lining. Neoplastic foci consisted of cuboidal and columnar cells that arranged in papillary and acinar forms. Proliferating cells appeared supported by loose connective tissue containing some fibroblasts and small blood vessels. Prominently, there was large number of swollen and ruffled macrophages in alveolar spaces adjacent to neoplastic foci. In conclusions, release of copious amount of mucoid fluid from nostrils and transformation of alveolar epithelial lining into cuboidal and columnar cells are pathognomonic for ovine pulmonary adenocarcinoma.

PDF

___

Bancroft JD, Stevens A, 1990. Theory and Practice of Histolo- gical Technique, third edition, Churchill Livingstone, Edin- burgh, Scotland, UK.
Cousens C, Thonur L, Imlach S, Crawford J, Sales J, Griffiths DJ, 2009. Jaagsiekte sheep retrovirus is present at high concentration in lung fluid produced by ovine pulmonary adenocarcinoma-affected sheep and can survive for seve- ral weeks at ambient temperatures. Res Vet Sci, 87, 154-156.
De las Heras M, González L, Sharp JM, 2003. Pathology of ovi- ne pulmonary adenocarcinoma. Curr Top Microbiol Immu- nol, 275, 25-54.
Fan H, Palmarini M, DeMartini JC, 2009. Transformation and oncogenesis by jaagsiekte sheep retrovirus. Curr Top Mic- robiol Immunol, 275, 139-177.
Grego E, De Meneghi D, Alvarez V, Benito AA, Minguijon E, Or- tin A, Mattoni M, Moreno B, Perez de Villarreal M, Alberti A, Capucchio MT, Caporale M, Juste R, Rosati S, De las Heras M, 2008. Colostrum and milk can transmit jaagsiekte retro- virus to lambs. Vet Microbiol, 130, 247-257.
Griffiths DJ, Martineau HM, Cousens C, 2010. Pathology and pathogenesis of ovine pulmonary adenocarcinoma. J Comp Pathol, 142, 260-283.
Kycko A, Reichert M, 2007. Current views on the mechanism of oncogenic cell transformation in ovine pulmonary ade- nocarcinoma. Postepy Hig Med Dosw, 61, 797-804.
Leroux C, Girard N, Cottin V, Greenland T, Mornex JF, Archer F, 2007. Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep. Vet Res, 38, 211-228.
Maeda N, Inoshima Y, Oouchi S, Uede T, 2011. Surveillance of Jaagsiekte sheep retrovirus in sheep in Hokkaido, the nort- hern island of Japan. J Vet Med Sci, 73, 1493-1495.
Minguijon E, Gonzalez L, De Las Heras M, Gomez N, Garcia- Goti M, Juste RA, Moreno B, 2013. Pathological and aetiolo- gical studies in sheep exhibiting extrathoracic metastasis of Ovine Pulmonary Adenocarcinoma (Jaagsiekte). J Comp Pathol, 148, 139-147.
Murgia C, Caporale M, Ceesay O, Di Francesco G, Ferri N, Vara- sano V, de las Heras M, Palmarini M, 2011. Lung adenocar- cinoma originates from retrovirus infection of prolifera- ting type 2 pneumocytes during pulmonary post-natal de- velopment or tissue repair. PLoS Pathog [Epub 2011]. doi: 10.1371/journal.ppat.1002014
Oda SS, Youssef SA, 2011. Immunohistochemical and histo- pathological findings of ovine pulmonary adenocarcinoma (Jaagsiekte) in Egyptian sheep. Trop Anim Health Prod, 43, 1611-1615.
Palmarini M, Fan H, 2001. Retrovirus-induced ovine pulmo- nary adenocarcinoma, an animal model for lung cancer. J Natl Cancer Inst, 93, 1603-1614.
Summers C, Norval M, De Las Heras M, Gonzalez L, Sharp JM, Woods GM, 2005. An influx of macrophages is the predo- minant local immune response in ovine pulmonary ade- nocarcinoma. Vet Immunol Immunopathol, 106, 285-294.
Wootton SK, Metzger MJ, Hudkins KL, Alpers CE, York D, De- Martini JC, Miller AD, 2006. Lung cancer induced in mice by the envelope protein of jaagsiekte sheep retrovirus (JSRV) closely resembles lung cancer in sheep infected with JSRV. Retrovirology, 3, 94-108.
York DF, Querat G, 2003. A history of ovine pulmonary adeno- carcinoma (jaagsiekte) and experiments leading to the de- duction of the JSRV nucleotide sequence. Curr Top Micro- biol Immunol, 275, 1-23.