Irmak DİK, Oguzhan AVCI, Sibel YAVRU, Kamil ATLI

Herpes Simplex Virus tip 1 inokule edilen Vero hücre kültüründe antioksidan enzim aktiviteleri

Amaç: Bu çalışma Herpes Simplex Virus tip 1 (HSV-1) ino- kule edilen Vero hücre kültürlerinde antioksidan kapasite/ oksidatif stres biomarkırlarını belirlemek amacı ile yapıldı. Gereç ve Yöntem: Vero hücre kültürüne HSV-1 inokule edilerek 21 saat boyunca (1. saate kadar 15 dkda bir, 6. saatekadar saatte bir, 6-10. saat arası 30 dkda bir, 10-21. saatarası saatte bir olmak üzere) hücre süpernatantları toplan- dı. Hücre süpernatantlarındaki süperoksit dismütaz (SOD),katalaz (CAT), glutasyon peroksidaz (GPX) enzimleri, glutasyon (GSH) ve malondialdehit (MDA) miktarı ticari ELISAkitleri ile ölçüldü. HSV-1in meydana getirdiği sitopatojeniketki (CPE) ise invert mikroskop yardımı ile periyodik olarakdeğerlendirildi.Bulgular: Araştırmada HSV-1 inokulasyonunu takiben 6. saatte CPE gözlendi. SODun en yüksek ve en düşük düzeyi sırasıyla 17. ve 1. saatte belirlenirken, 18. saatte CAT ve 7. saatteGPX seviyeleri en düşük düzeyde tespit edildi. GSH miktarıilk 30 dk içinde minimum seviyeye ulaştıktan sonra 10. saatte miktarında artış meydana geldi. MDA miktarında 3. saatteani yükseliş olurken 18. saatte maksimum seviyeye ulaştığıbelirlendi. Oksidatif stresin önemli belirteçleri olan SOD veGSH düzeylerinin HSV-1in neden olduğu CPE oluşumundanönce azaldığı tespit edildi. Öneri: Lipid peroksidasyonunun HSV-1in patogenezinde rolalabileceği ve HSV-1 enfeksiyonu tedavisinde antioksidan uygulamalarının faydalı olabileceği ifade edilebilir.

Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1.

Aim: The aim of the present study was to determine of antioxidant capacity/oxidative stress biomarkers in Vero cell lineinoculated with Herpes Simplex Virus type 1 (HSV-1). Materials and Methods: Cell supernatants were collectedduring 21 hours (15 min interval at first hour, 1 hour interval by 6th hour, 30 min interval at 6th -10th hours, 1 hourinterval at 6th-21th hour) after HSV-1 inoculations. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) enzymes, glutathione (GSH) and malondialdehyde(MDA) values were analyzed from the test media by commer- cially available ELISA kits. In addition to this, cytopathogeniceffects (CPE) of HSV-1 in cell culture were evaluated periodically by invert microscope. Results: In this research, CPE of HSV-1 was observed at 6thhour post-inoculation. Maximum and minimum levels of SODwere determined at 17th and 1st hours, respectively. Minimum levels of CAT and GPX were determined at 18th and 7thhour, respectively. Minimum level of GSH was determined atfirst 30 minutes whereas maximum level of GSH was measured at 10th hours. MDA levels suddenly increased at 3rd hours and MDA reached maximum level at 18th hour. The SODand GSH levels, important markers of oxidative stress, werereduced on HSV-1 before the formation CPE. Conclusion: It is concluded that lipid peroxidation may occur in the pathogenesis of HSV-1 and antioxidant treatmentmay be useful in the therapy of HSV-1 infection.

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