Fatih FİDAN, H. Ahmet CELİK, Gulcan AVCİ, Recep ASLAN, Aziz BULBUL

Effects of ovarian steroids on oxidative stress in overiectomized rats

Amaç: Ovariektomi yapılmış ratlarda östrojen ve progesteron hormonlarının beraber veya ayrı uygulanmasının lipid peroksidasyon, antioksidatif aktivite (AOA) ile β-karoten, vitamin A ve C düzeyine olan etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Araştırma overioktemi yapılmış ve her biri 10 rattan oluşan kontrol ve 3 deneme grubunda yürütüldü. Kontrol grubuna susam yağı, progesteron grubuna progesteron 2 mg/rat/gün, östrojen grubuna 17β-östradiol 10 mg/rat/gün, östrojen + progesteron grubuna 17β-östradiol 10 mg/rat/gün + progestagen 2 mg/rat/gün olarak 10 gün uygulandı. En son uygulamadan 2 saat sonra genel anestezi altındaki ratlardan alınan kan örneklerinde malondialdehit (MDA), AOA, β-karoten, vitamin A ve C düzeyleri ölçüldü. Bulgular: Tam kan MDA düzeylerinin kontrol gruba göre östrojen grubunda azaldığı, ancak diğer deneme gruplarında fark bulunmadığı tespit edildi. Plazma AOA düzeyinin östrojen ve östrojen + progesteron gruplarında kontrol ve progesteron gruplarına göre yüksek (p

Ovariektomize ratlarda ovaryum steroidlerinin oksidatif stres üzerine etkisi.

Aim: Determining the effects of combined or separate estrogen and progesterone administration on lipid peroxidation, antioxidative activity (AOA), β-carotene, vitamin A and C levels in overiectomized rats. Materials and Methods: The study was conducted on 1 control and 3 trial groups each consisted of 10 overiectomized rats. Control group received sesame oil while progesterone group received 2 mg/rat/day progesterone, estrogen group 10 mg/rat/day 17β-estradiol and estrogen + progesteron group 10 mg/rat/day 17β-estradiol+ 2 mg/rat/ day progesterone for 10 days. Blood samples of rats were collected under general anesthesia after 2 hours following last administration and checked for malondialdehyde (MDA), AOA, β-carotene, vitamin A, and C levels. Results: Whole blood MDA levels in estrogen group were decreased as compared to control group while remained same in others. Plasma AOA levels of estrogen and estrogen + progesterone groups were higher (p

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