Acute phase response and clinical changes in calves with lipopolysaccharide induced endotoxemia

Amaç: Lipopolisakkarit ile deneysel endotoksemi oluşturulan buzağılarda akut faz proteinlerin ve klinik değişimlerin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada yaşları 25-42 gün arasında değişen sekiz adet Holstein ırkı buzağı kullanıldı. Lipopolisakkarit damar içi 0.1 μg/kg dozunda 50 mL % 0.9 NaCl’de seyreltilip 30 dakika süresince verilerek endotoksemi oluşturuldu. Çalışma boyunca endotoksemi gelişen buzağılarda klinik olarak gözlenen değişiklikler kayıt edilirken ve akut faz proeinlerinin konsantrasyonları belirlendi. Bulgular: Lipopolisakkarit uygulanması sonrasında buzağıların tamamında solunum sayısında ve kalp atım sayısında artış, yerde yatma, depresyon, hipotermi/hipertermi mukozada hiperemi/siyanoz ve emme refleksinde azalma gözlendi. Klinik olarak gelişen değişimler 1. ve 3. saatler arasında daha şiddetli olduğu belirlendi. Damar içi lipopolisakkarit verilmesinden sonra haptoglobin 36. saatte (500±93.2 μg/ mL) ve serum amyloid-A 24. saatte (185± 46.6 μg/mL) en üst seviyeye ulaştı. Öneri: Akut faz proteinlerde ve klinik bulgulardaki gözlenen değişim, buzağılarda gelişen endotoksemi ile ilişkilidir. Buzağılarda serum haptoglobin ve serum amyloid-A konsantrasyonlardaki artışlar endotokseminin bir belirteçi olarak değerlendirilebilir.

Lipopolisakkarit ile endotoksemi oluşturulan buzağılarda akut faz cevap ve klinik değişimler

Aim: The purpose of the study was to determine the levels of acute phase proteins and clinical changes in calves with lipopolysaccharide induced experimental endotoxemia. Materials and Methods: Eight Holstein breed calves were used in the study. Endotoxemia was induced via intravenous administration of 0.1 μg/kg dose of lipopolysaccharide in 50 mL of physiological 0.9% NaCl over 30 min. The calves were continuously observed for clinical changes during the experiment, and serum acute phase protein levels were measured. Results: Increase in respiratory frequency, tachycardi, mucosal hyperemia/cyanosis, recumbency, depression, hyperthermia/hypothermia, and poor suckle reflexes were observed in all calves after lipopolysaccharide administration. After lipopolysaccharide infusion, serum haptoglobin (500±93.2 μg/mL) and serum amyloid A (185±46.6) concentrations reached peak levels of at 36 h and 24 h, respectively. Conclusion: Changes in acute phase proteins and clinical findings were related to endotoxemia. A moderate to high increase in haptoglobin and serum amyloid A concentrations may indicate the presence of endotoxemia in calves.

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